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Probing Glioblastoma Tissue Heterogeneity with Laser Capture Microdissection.

Authors :
Gagner JP
Zagzag D
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1741, pp. 209-220.
Publication Year :
2018

Abstract

Among various methods now available to isolate distinct cell populations or even single cells for DNA/RNA and proteomic analysis, laser capture microdissection (LCM) offers a unique opportunity to study cells in their topological contexts. This chapter focuses on the preparation of LCM membrane slides, tissue staining and laser microdissection of cells of interest from frozen or formalin-fixed, paraffin-embedded glioblastoma tissue.

Subjects

Subjects :
Gene Expression Profiling methods
Glioblastoma genetics
Glioblastoma metabolism
Humans
Immunohistochemistry
Paraffin Embedding
Staining and Labeling
Tissue Fixation
Glioblastoma pathology
Laser Capture Microdissection methods

Details

Language :
English
ISSN :
1940-6029
Volume :
1741
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
29392703
Full Text :
https://doi.org/10.1007/978-1-4939-7659-1_17
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