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Multifunctional Tannic Acid/Silver Nanoparticle-Based Mucoadhesive Hydrogel for Improved Local Treatment of HSV Infection: In Vitro and In Vivo Studies.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2018 Jan 28; Vol. 19 (2). Date of Electronic Publication: 2018 Jan 28. - Publication Year :
- 2018
-
Abstract
- Mucoadhesive gelling systems with tannic acid modified silver nanoparticles were developed for effective treatment of herpes virus infections. To increase nanoparticle residence time after local application, semi solid formulations designed from generally regarded as safe (GRAS) excipients were investigated for their rheological and mechanical properties followed with ex vivo mucoadhesive behavior to the porcine vaginal mucosa. Particular effort was made to evaluate the activity of nanoparticle-based hydrogels toward herpes simplex virus (HSV) type 1 and 2 infection in vitro in immortal human keratinocyte cell line and in vivo using murine model of HSV-2 genital infection. The effect of infectivity was determined by real time quantitative polymerase chain reaction, plaque assay, inactivation, attachment, penetration and cell-to-cell assessments. All analyzed nanoparticle-based hydrogels exhibited pseudoplastic and thixotropic properties. Viscosity and mechanical measurements of hydrogels were found to correlate with the mucoadhesive properties. The results confirmed the ability of nanoparticle-based hydrogels to affect viral attachment, impede penetration and cell-to-cell transmission, although profound differences in the activity evoked by tested preparations toward HSV-1 and HSV-2 were noted. In addition, these findings demonstrated the in vivo potential of tannic acid modified silver nanoparticle-based hydrogels for vaginal treatment of HSV-2 genital infection.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Adhesives chemistry
Animals
Antiviral Agents administration & dosage
Antiviral Agents therapeutic use
Cell Line
Chlorocebus aethiops
Female
Humans
Hydrogel, Polyethylene Glycol Dimethacrylate chemistry
Hydrogel, Polyethylene Glycol Dimethacrylate therapeutic use
Metal Nanoparticles chemistry
Mice
Mice, Inbred C57BL
Silver chemistry
Swine
Tannins administration & dosage
Tannins therapeutic use
Antiviral Agents pharmacology
Herpes Simplex drug therapy
Metal Nanoparticles therapeutic use
Simplexvirus drug effects
Tannins pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 19
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 29382085
- Full Text :
- https://doi.org/10.3390/ijms19020387