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Platelet-predominate gene expression and reticulated platelets in nonvalvular atrial fibrillation: Effect of pulmonary veins isolation.
- Source :
-
Journal of cardiovascular electrophysiology [J Cardiovasc Electrophysiol] 2018 Mar; Vol. 29 (3), pp. 412-420. Date of Electronic Publication: 2018 Feb 13. - Publication Year :
- 2018
-
Abstract
- Introduction: Reticulated platelet (RP) content is increased in nonvalvular atrial fibrillation (NVAF). The purpose of this study was to determine if platelet content, morphology, and RP proportion are modulated by platelet genes.<br />Methods and Results: Expression of six platelet-predominate genes impacting platelet formation and release, platelet count, and RP content was assessed in NVAF patients before and 3-4 months after pulmonary veins isolation (PVI) and compared to normal sinus rhythm (NSR) controls. RNA from isolated platelets was reverse-transcribed assayed against selected genes utilizing real-time qPCR, and expressed as mean cycle threshold (ΔCt) using beta-2-microglobulin as endogenous control. RP content was assessed by flow cytometry. A fourfold lower expression of CFL1 gene coding for nonmuscle cofilin (7.8 ± 0.9 vs. 5.7 ± 1.6, P < 0.001) and twofold lower expression of four other genes were associated with similar platelet counts but fourfold higher (28.7+7.0 vs. 6.7+5.4, P < 0.001) RP content (%) in 97 NVAF cases compared to 51 NSR controls. Three to 4 months after PVI, RP decreased by 28%, while CFL1 gene expression increased over twofold but TUBA4A gene expression decreased almost twofold; NFE2 and MYL6 gene expression remained unchanged.<br />Conclusions: NVAF is associated with notable downregulation of genes directing platelet production and size but increased RP content. PVI impacts the expression of many of these genes, implying a direct relationship between atrial fibrillation and platelet biogenesis.<br /> (© 2018 Wiley Periodicals, Inc.)
- Subjects :
- Action Potentials
Aged
Atrial Fibrillation blood
Atrial Fibrillation diagnosis
Atrial Fibrillation physiopathology
Blood Platelets pathology
Case-Control Studies
Cofilin 1 blood
Cofilin 1 genetics
Female
Gene Expression Regulation
Heart Rate
Humans
Male
Membrane Proteins blood
Membrane Proteins genetics
Middle Aged
Myosin Light Chains blood
Myosin Light Chains genetics
NF-E2 Transcription Factor, p45 Subunit blood
NF-E2 Transcription Factor, p45 Subunit genetics
Platelet Count
Pulmonary Veins physiopathology
Receptors, Progesterone blood
Receptors, Progesterone genetics
Time Factors
Treatment Outcome
Tubulin blood
Tubulin genetics
Atrial Fibrillation surgery
Blood Platelets metabolism
Catheter Ablation adverse effects
Pulmonary Veins surgery
Subjects
Details
- Language :
- English
- ISSN :
- 1540-8167
- Volume :
- 29
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of cardiovascular electrophysiology
- Publication Type :
- Academic Journal
- Accession number :
- 29377394
- Full Text :
- https://doi.org/10.1111/jce.13438