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Increased ceruloplasmin expression caused by infiltrated leukocytes, activated microglia, and astrocytes in injured female rat spinal cords.
- Source :
-
Journal of neuroscience research [J Neurosci Res] 2018 Jul; Vol. 96 (7), pp. 1265-1276. Date of Electronic Publication: 2018 Jan 29. - Publication Year :
- 2018
-
Abstract
- Ceruloplasmin (Cp), an enzyme containing six copper atoms, has important roles in iron homeostasis and antioxidant defense. After spinal cord injury (SCI), the cellular components in the local microenvironment are very complex and include functional changes of resident cells and the infiltration of leukocytes. It has been confirmed that Cp is elevated primarily in astrocytes and to a lesser extent in macrophages following SCI in mice. However, its expression in other cell types is still not very clear. In this manuscript, we provide a sensible extension of these findings by examining this system within a female Sprague-Dawley rat model and expanding the scope of inquiry to include additional cell types. Quantitative reverse transcription polymerase chain reaction and Western blot analysis revealed that the Cp mRNA and protein in SCI tissue homogenates were quite consistent with prior publications. However, we observed that Cp was expressed not only in GFAP <superscript>+</superscript> astrocytes (consistent with prior reports) but also in CD11b <superscript>+</superscript> microglia, CNPase <superscript>+</superscript> oligodendrocytes, NeuN <superscript>+</superscript> neurons, CD45 <superscript>+</superscript> leukocytes, and CD68 <superscript>+</superscript> activated microglia/macrophages. Quantitative analysis proved that infiltrated leukocytes, activated microglia/macrophages, and astrocytes should be the major sources of increased Cp.<br /> (© 2018 Wiley Periodicals, Inc.)
- Subjects :
- 2',3'-Cyclic-Nucleotide Phosphodiesterases metabolism
Animals
Antigens, CD metabolism
Antigens, Differentiation, Myelomonocytic metabolism
Antigens, Nuclear metabolism
Astrocytes pathology
CD11b Antigen metabolism
Ceruloplasmin metabolism
Female
Glial Fibrillary Acidic Protein metabolism
Leukocyte Common Antigens metabolism
Leukocytes enzymology
Leukocytes pathology
Macrophages enzymology
Macrophages pathology
Mice
Microglia pathology
Nerve Tissue Proteins metabolism
Neurons enzymology
Neurons physiology
Oligodendroglia enzymology
Oligodendroglia pathology
Rats
Rats, Sprague-Dawley
Spinal Cord Injuries chemically induced
Astrocytes enzymology
Ceruloplasmin biosynthesis
Microglia enzymology
Spinal Cord Injuries pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4547
- Volume :
- 96
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of neuroscience research
- Publication Type :
- Academic Journal
- Accession number :
- 29377294
- Full Text :
- https://doi.org/10.1002/jnr.24221