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Acetaminophen and Metamizole Induce Apoptosis in HT 29 and SW 480 Colon Carcinoma Cell Lines In Vitro .
- Source :
-
Anticancer research [Anticancer Res] 2018 Feb; Vol. 38 (2), pp. 745-751. - Publication Year :
- 2018
-
Abstract
- Background/aim: The perioperative phase is supposed to be a period with high vulnerability for cancer dissemination. Acetaminophen and metamizole are common analgesics administered during this phase. We investigated the effect of acetaminophen, metamizole and 4-methylaminoantipyrine (MAA) on proliferation and apoptosis of colon carcinoma cell lines (SW 480 and HT 29).<br />Materials and Methods: Proliferation was detected by cell proliferation ELISA BrdU, and apoptosis by Annexin V staining. Cytochrome c and caspase 3, 8 and 9 expression levels were detected by western blot.<br />Results: Acetaminophen, metamizole or MAA caused slight changes in proliferation. Acetaminophen, metamizole or the combination increased apoptosis in both cell lines. All agents decreased caspase 3 and 8 expression in SW480. Acetaminophen decreased caspase 9 expression in both cell lines.<br />Conclusion: In clinically relevant doses, acetaminophen and/or metamizole induce apoptosis in both colon cancer cell lines. Both mitochondrial and death receptor pathways might be involved in acetaminophen-induced apoptosis.<br /> (Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Subjects :
- Aminopyrine analogs & derivatives
Aminopyrine pharmacology
Anti-Inflammatory Agents, Non-Steroidal pharmacology
Blotting, Western
Cell Line, Tumor
Cell Proliferation drug effects
Colonic Neoplasms pathology
HT29 Cells
Humans
Acetaminophen pharmacology
Analgesics, Non-Narcotic pharmacology
Apoptosis drug effects
Colonic Neoplasms drug therapy
Dipyrone pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-7530
- Volume :
- 38
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Anticancer research
- Publication Type :
- Academic Journal
- Accession number :
- 29374698
- Full Text :
- https://doi.org/10.21873/anticanres.12280