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Immunization with a Mixture of Nucleoprotein from Human Metapneumovirus and AbISCO-100 Adjuvant Reduces Viral Infection in Mice Model.
- Source :
-
Viral immunology [Viral Immunol] 2018 May; Vol. 31 (4), pp. 306-314. Date of Electronic Publication: 2018 Jan 26. - Publication Year :
- 2018
-
Abstract
- The human metapneumovirus (hMPV) is the second leading cause globally of acute infection of the respiratory tract in children, infecting the upper and lower airways. The hMPV may induce an inappropriate Th2-type immune response, which causes severe pulmonary inflammation, leading to the obstruction of airways. Despite its severe epidemiological relevance, no vaccines are currently available for the prevention of hMPV-induced illness. In this investigation, we demonstrated that immunization of mice with the recombinant hMPV nucleoprotein (hMPV-N) mixed with the AbISCO-100 adjuvant reduced viral replication in lungs following challenge with the virus. We found that immunized mice had reduced weight loss, decreased granulocytes in the lung, an increased level of specific nucleoprotein antibodies of IgG1 and IgG2a-isotypes, and a local profile of Th1/Th17-type cytokines. Our results suggest that immunization with the hMPV-N and the AbISCO-100 adjuvant induces a reduction of viral infection and could be considered for the development of an hMPV vaccine.
- Subjects :
- Adjuvants, Immunologic pharmacology
Animals
Antibodies, Viral blood
Antibodies, Viral classification
Cytokines analysis
Dendritic Cells classification
Disease Models, Animal
Gene Expression drug effects
Granulocytes
Humans
Lung drug effects
Lung pathology
Lung virology
Mice
Mice, Inbred BALB C
Nucleoproteins genetics
Nucleoproteins isolation & purification
Paramyxoviridae Infections prevention & control
Pneumonia virology
RNA, Viral analysis
Viral Vaccines pharmacology
Weight Loss
Immunization
Metapneumovirus immunology
Nucleoproteins administration & dosage
Paramyxoviridae Infections immunology
Viral Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1557-8976
- Volume :
- 31
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Viral immunology
- Publication Type :
- Academic Journal
- Accession number :
- 29373084
- Full Text :
- https://doi.org/10.1089/vim.2017.0159