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[Enzymologic study of the structural organization of the matrix or rat liver peroxisomes].
- Source :
-
Tsitologiia [Tsitologiia] 1978 Jun; Vol. 20 (6), pp. 676-81. - Publication Year :
- 1978
-
Abstract
- The effect of ionic strength and pH on the release of some enzymes of the matrix of peroxisomes in rat's liver was studied. Catalase, L ALpha-hydroxy acid oxidase, isocitrate dehydrogenase, glycerophosphate dehydrogenase and lactate dehydrogenase were easily released from the particles during their lysis and treatment with 0.16 M KCl, whereas urate oxidase, NADH cytochrome c reductase and D-amino acid oxidase were not solubilized. After the solubilization of peroxisomal membrane by 0.2% Triton X-100, the remaining core contained about 50% amino acid oxidase activity, and had 1.28--1.30 g/cm3 density. These results suggest that D-amino acid oxidase associates with urate oxidase in the peroxisomal core.
- Subjects :
- Amino Acid Oxidoreductases metabolism
Animals
Catalase metabolism
Glycerolphosphate Dehydrogenase metabolism
Hydrogen-Ion Concentration
Isocitrate Dehydrogenase metabolism
L-Lactate Dehydrogenase metabolism
Male
Microbodies ultrastructure
NADPH-Ferrihemoprotein Reductase metabolism
Osmolar Concentration
Oxidoreductases metabolism
Rats
Urate Oxidase metabolism
Liver ultrastructure
Microbodies enzymology
Organoids enzymology
Subjects
Details
- Language :
- Russian
- ISSN :
- 0041-3771
- Volume :
- 20
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Tsitologiia
- Publication Type :
- Academic Journal
- Accession number :
- 29368