OMIP-044: 28-color immunophenotyping of the human dendritic cell compartment.

Authors :: Mair F
Prlic M
Source :: Cytometry. Part A : the journal of the International Society for Analytical Cytology [Cytometry A] 2018 Apr; Vol. 93 (4), pp. 402-405. Date of Electronic Publication: 2018 Jan 22.
Publication Year :: 2018
Abstract: This work describes the first 30-parameter immunophenotyping of the human dendritic cell (DC) compartment using fluorescent-based flow cytometry. The optimized panel allows for simultaneous detection of 21 myeloid-centric markers distinguishing all canonical DC subsets, with parallel enumeration of monocytes, T and B cells as well as NK cells. Thus, this panel will be useful for extensive phenotyping of immune cells from a variety of human samples limited in size.<br /> (© 2018 International Society for Advancement of Cytometry.)

Subjects :: B-Lymphocytes metabolism
Biomarkers metabolism
Color
Flow Cytometry methods
Humans
Immunophenotyping methods
Killer Cells, Natural metabolism
Monocytes metabolism
Myeloid Cells metabolism
T-Lymphocytes metabolism
Dendritic Cells metabolism

Language :: English
ISSN :: 1552-4930
Volume :: 93
Issue :: 4
Database :: MEDLINE
Journal :: Cytometry. Part A : the journal of the International Society for Analytical Cytology
Publication Type :: Academic Journal
Accession number :: 29356334
Full Text :: https://doi.org/10.1002/cyto.a.23331