Therapeutic effect of Lipoxin A 4  in malaria-induced acute lung injury.

Acute lung injury (ALI) models are characterized by neutrophil accumulation, tissue damage, alteration of the alveolar capillary membrane, and physiological dysfunction. Lipoxin A ₄  (LXA ₄ ) is an anti-inflammatory eicosanoid that was demonstrated to attenuate lipopolysaccharide-induced ALI. Experimental models of severe malaria can be associated with lung injury. However, to date, a putative effect of LXA ₄  on malaria (M)-induced ALI has not been addressed. In this study, we evaluated whether LXA ₄ exerts an effect on M-ALI. Male C57BL/6 mice were randomly assigned to the following five groups: noninfected; saline-treated Plasmodium berghei-infected; LXA ₄ -pretreated P. berghei-infected (LXA ₄  administered 1 h before infection and daily, from days 0 to 5 postinfection), LXA ₄ - and LXA ₄ receptor antagonist BOC-2-pretreated P. berghei-infected; and LXA ₄ -posttreated P. berghei-infected (LXA ₄  administered from days 3 to 5 postinfection). By day 6, pretreatment or posttreatment with LXA ₄  ameliorate lung mechanic dysfunction reduced alveolar collapse, thickening and interstitial edema; impaired neutrophil accumulation in the pulmonary tissue and blood; and reduced the systemic production of CXCL1. Additionally, in vitro treatment with LXA ₄ prevented neutrophils from migrating toward plasma collected from P. berghei-infected mice. LXA ₄  also impaired neutrophil cytoskeleton remodeling by inhibiting F-actin polarization. Ex vivo analysis showed that neutrophils from pretreated and posttreated mice were unable to migrate. In conclusion, we demonstrated that LXA ₄  exerted therapeutic effects in malaria-induced ALI by inhibiting lung dysfunction, tissue injury, and neutrophil accumulation in lung as well as in peripheral blood. Furthermore, LXA ₄ impaired the migratory ability of P. berghei-infected mice neutrophils.
(©2018 Society for Leukocyte Biology.)