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Characterization of CD34 + hematopoietic cells in systemic mastocytosis: Potential role in disease dissemination.
- Source :
-
Allergy [Allergy] 2018 Jun; Vol. 73 (6), pp. 1294-1304. Date of Electronic Publication: 2018 Feb 13. - Publication Year :
- 2018
-
Abstract
- Background: Recent studies show that most systemic mastocytosis (SM) patients, including indolent SM (ISM) with (ISMs+) and without skin lesions (ISMs-), carry the KIT D816V mutation in PB leukocytes. We investigated the potential association between the degree of involvement of BM hematopoiesis by the KIT D816V mutation and the distribution of different maturation-associated compartments of bone marrow (BM) and peripheral blood (PB) CD34 <superscript>+</superscript> hematopoietic precursors (HPC) in ISM and identified the specific PB cell compartments that carry this mutation.<br />Methods: The distribution of different maturation-associated subsets of BM and PB CD34 <superscript>+</superscript> HPC from 64 newly diagnosed (KIT-mutated) ISM patients and 14 healthy controls was analyzed by flow cytometry. In 18 patients, distinct FACS-purified PB cell compartments were also investigated for the KIT mutation.<br />Results: ISM patients showed higher percentages of both BM and PB MC-committed CD34 <superscript>+</superscript> HPC vs controls, particularly among ISM cases with MC-restricted KIT mutation (ISM <subscript>MC</subscript> ); this was associated with progressive blockade of maturation of CD34 <superscript>+</superscript> HPC to the neutrophil lineage from ISM <subscript>MC</subscript> to multilineage KIT-mutated cases (ISM <subscript>ML</subscript> ). Regarding the frequency of KIT-mutated cases and cell populations in PB, variable patterns were observed, the percentage of KIT-mutated PB CD34 <superscript>+</superscript> HPC, eosinophils, neutrophils, monocytes and T cells increasing from ISMs- <subscript>MC</subscript> and ISMs+ <subscript>MC</subscript> to ISM <subscript>ML</subscript> patients.<br />Conclusion: The presence of the KIT D816V mutation in PB of ISM patients is associated with (early) involvement of circulating CD34 <superscript>+</superscript> HPC and multiple myeloid cell subpopulations, KIT-mutated PB CD34 <superscript>+</superscript> HPC potentially contributing to early dissemination of the disease.<br /> (© 2018 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.)
- Subjects :
- Alleles
Antigens, CD34 metabolism
Biomarkers
Bone Marrow Cells cytology
Bone Marrow Cells metabolism
Case-Control Studies
Cell Differentiation genetics
Female
Genotype
Hematopoietic Stem Cells cytology
Humans
Immunophenotyping
Leukocytes cytology
Leukocytes metabolism
Male
Mastocytosis, Systemic diagnosis
Mutation
Proto-Oncogene Proteins c-kit genetics
Proto-Oncogene Proteins c-kit metabolism
Spain
Hematopoietic Stem Cells metabolism
Mastocytosis, Systemic etiology
Mastocytosis, Systemic metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1398-9995
- Volume :
- 73
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Allergy
- Publication Type :
- Academic Journal
- Accession number :
- 29331029
- Full Text :
- https://doi.org/10.1111/all.13413