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Cranberry proanthocyanidin-chitosan hybrid nanoparticles as a potential inhibitor of extra-intestinal pathogenic Escherichia coli invasion of gut epithelial cells.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2018 May; Vol. 111, pp. 415-420. Date of Electronic Publication: 2018 Jan 08. - Publication Year :
- 2018
-
Abstract
- Chitosan interacts with proanthocyanidins through hydrogen-bonding, which allows encapsulation and development of stable nanoparticles via ionotropic gelation. Cranberry proanthocyanidins (PAC) are associated with the prevention of urinary tract infections and PAC inhibit invasion of gut epithelial cells by extra-intestinal pathogenic Escherichia coli (ExPEC). We determined the effect of cranberry proanthocyanidin-chitosan hybrid nanoparticles (PAC-CHTNp) on the ExPEC invasion of gut epithelial cells in vitro. PAC-CHTNp were characterized according to size, morphology, and bioactivity. Results showed a decrease in the size of the nanoparticles as the concentration of PAC was increased, indicating that PAC increases cross-linking by hydrogen-bonding on the surface of the chitosan nanoparticles. Nanoparticles were produced with diameters ranging from 367.3 nm to 293.2 nm. Additionally, PAC-CHTNp significantly inhibited the ability of ExPEC to invade the enterocytes by ~80% at 66 μg GAE/mL and by ~92% at 100 μg GAE/mL. Results also indicate that chitosan nanoparticles alone were not significantly different from controls in preventing ExPEC invasion of enterocytes (data not shown) and also there were not significant differences between PAC alone and PAC-CHTNp, suggesting that the new PAC-CHTNp could lead to an increase in the stability of encapsulated PAC, maintain the molecular adhesion of PAC to ExPEC.<br /> (Published by Elsevier B.V.)
- Subjects :
- Chitosan chemistry
Epithelial Cells microbiology
Escherichia coli pathogenicity
Humans
Intestines drug effects
Intestines microbiology
Nanoparticles chemistry
Proanthocyanidins pharmacology
Epithelial Cells drug effects
Escherichia coli drug effects
Proanthocyanidins chemistry
Vaccinium macrocarpon chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 111
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 29325748
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2018.01.033