Back to Search
Start Over
In Vivo Infection Model of Severe Influenza A Virus.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1725, pp. 91-99. - Publication Year :
- 2018
-
Abstract
- The lung is constantly exposed to both environmental and microbial challenge. As a "contained" organ, it also constitutes an excellent "self-contained" tissue to examine inflammatory responses and cellular infiltration into a diseased organ. Influenza A virus (IAV) causes both mild and severe inflammation that is strain specific following infection of the lung epithelium that spreads to other cells of the lung environment. Here, we describe a method of intranasal inoculation of the lung with IAV that can be used as a preclinical model of infection. Mice can be monitored for clinical signs of infection and tissue and lung fluid collected for further analysis to dissect the immunological consequences of IAV infection. Importantly, this method can be modified to introduce other pathogens, therapies and environmental stimuli to examine immune responses in the lung.
- Subjects :
- Administration, Intranasal
Animals
Bronchoalveolar Lavage Fluid microbiology
Cytokines metabolism
Female
Lung metabolism
Lung microbiology
Male
Mice
Mice, Inbred C57BL
Orthomyxoviridae Infections microbiology
Pneumonia pathology
Bronchoalveolar Lavage Fluid immunology
Disease Models, Animal
Influenza A virus pathogenicity
Lung immunology
Orthomyxoviridae Infections complications
Pneumonia etiology
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1725
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 29322411
- Full Text :
- https://doi.org/10.1007/978-1-4939-7568-6_8