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Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility.
- Source :
-
The New phytologist [New Phytol] 2018 Apr; Vol. 218 (1), pp. 81-93. Date of Electronic Publication: 2018 Jan 08. - Publication Year :
- 2018
-
Abstract
- Feruloylation of arabinoxylan (AX) in grass cell walls is a key determinant of recalcitrance to enzyme attack, making it a target for improvement of grass crops, and of interest in grass evolution. Definitive evidence on the genes responsible is lacking so we studied a candidate gene that we identified within the BAHD acyl-CoA transferase family. We used RNA interference (RNAi) silencing of orthologs in the model grasses Setaria viridis (SvBAHD01) and Brachypodium distachyon (BdBAHD01) and determined effects on AX feruloylation. Silencing of SvBAHD01 in Setaria resulted in a c. 60% decrease in AX feruloylation in stems consistently across four generations. Silencing of BdBAHD01 in Brachypodium stems decreased feruloylation much less, possibly due to higher expression of functionally redundant genes. Setaria SvBAHD01 RNAi plants showed: no decrease in total lignin, approximately doubled arabinose acylated by p-coumarate, changes in two-dimensional NMR spectra of unfractionated cell walls consistent with biochemical estimates, no effect on total biomass production and an increase in biomass saccharification efficiency of 40-60%. We provide the first strong evidence for a key role of the BAHD01 gene in AX feruloylation and demonstrate that it is a promising target for improvement of grass crops for biofuel, biorefining and animal nutrition applications.<br /> (© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.)
- Subjects :
- Acids metabolism
Brachypodium genetics
Carbohydrate Metabolism
Coenzyme A-Transferases metabolism
Gene Expression Regulation, Plant
Gene Silencing
Hydrolysis
Lignin metabolism
Magnetic Resonance Spectroscopy
Organ Size
Phylogeny
Plant Stems metabolism
Plants, Genetically Modified
Seeds anatomy & histology
Seeds growth & development
Transcriptome genetics
Xylans metabolism
Biomass
Cell Wall metabolism
Coenzyme A-Transferases genetics
Coumaric Acids metabolism
Genes, Plant
Setaria Plant enzymology
Setaria Plant genetics
Suppression, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8137
- Volume :
- 218
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The New phytologist
- Publication Type :
- Academic Journal
- Accession number :
- 29315591
- Full Text :
- https://doi.org/10.1111/nph.14970