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Comparison between two inhibin B ELISA assays in 46,XY testicular disorders of sex development (DSD) with normal testosterone secretion.
- Source :
-
Journal of pediatric endocrinology & metabolism : JPEM [J Pediatr Endocrinol Metab] 2018 Jan 26; Vol. 31 (2), pp. 191-194. - Publication Year :
- 2018
-
Abstract
- Background: Inhibin B is a hormone produced by the Sertoli cells that can provide important information for the investigation of disorders of sex development (DSD) with 46,XY karyotype. The aim of this study is to compare two enzyme-linked immunosorbent assay (ELISA) assays for dosage of serum inhibin B in patients with 46,XY DSD with normal testosterone secretion.<br />Methods: Twenty-nine patients with 46,XY DSD and normal testosterone secretion (partial androgen insensitivity syndrome [PAIS] [n=8]; 5α-reductase deficiency [n=7] and idiopathic 46,XY DSD [n=14]) were included. Molecular analysis of the AR and SRD5A2 genes were performed in all patients and the NR5A1 gene analysis in the idiopathic group. Measurements of inhibin B were performed by two second-generation ELISA assays (Beckman-Coulter and AnshLabs). Assays were compared using the interclass correlation coefficient (ICC) and the Bland-Altman method.<br />Results: ICC was 0.915 [95% confidence interval (CI): 0.828-0.959], however, a discrepancy was observed between trials, which is more evident among higher values when analyzed by the Bland-Altman method.<br />Conclusions: It is recommended to perform the inhibin B measurement always using the same ELISA kit when several evaluations are required for a specific patient.
- Subjects :
- 3-Oxo-5-alpha-Steroid 4-Dehydrogenase blood
3-Oxo-5-alpha-Steroid 4-Dehydrogenase deficiency
3-Oxo-5-alpha-Steroid 4-Dehydrogenase genetics
Adolescent
Adult
Androgen-Insensitivity Syndrome blood
Androgen-Insensitivity Syndrome diagnosis
Androgen-Insensitivity Syndrome genetics
Androgen-Insensitivity Syndrome physiopathology
Child
Child, Preschool
Diagnosis, Differential
Disorder of Sex Development, 46,XY diagnosis
Disorder of Sex Development, 46,XY genetics
Disorder of Sex Development, 46,XY physiopathology
Hospitals, University
Humans
Hypospadias blood
Hypospadias diagnosis
Hypospadias genetics
Hypospadias physiopathology
Karyotype
Male
Membrane Proteins genetics
Outpatient Clinics, Hospital
Receptors, Androgen genetics
Reproducibility of Results
Severity of Illness Index
Steroid Metabolism, Inborn Errors blood
Steroid Metabolism, Inborn Errors diagnosis
Steroid Metabolism, Inborn Errors genetics
Steroid Metabolism, Inborn Errors physiopathology
Steroidogenic Factor 1 genetics
Testis physiopathology
Young Adult
Disorder of Sex Development, 46,XY blood
Enzyme-Linked Immunosorbent Assay
Inhibin-beta Subunits blood
Testis metabolism
Testosterone metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2191-0251
- Volume :
- 31
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of pediatric endocrinology & metabolism : JPEM
- Publication Type :
- Academic Journal
- Accession number :
- 29306929
- Full Text :
- https://doi.org/10.1515/jpem-2017-0351