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pH-sensitive polymeric nanoparticles of mPEG-PLGA-PGlu with hybrid core for simultaneous encapsulation of curcumin and doxorubicin to kill the heterogeneous tumour cells in breast cancer.
- Source :
-
Artificial cells, nanomedicine, and biotechnology [Artif Cells Nanomed Biotechnol] 2018; Vol. 46 (sup1), pp. 302-313. Date of Electronic Publication: 2018 Jan 04. - Publication Year :
- 2018
-
Abstract
- Most breast tumours are heterogeneous and not only contain the bulk of differentiated tumour cells but also a small population of highly tumorigenic and intrinsically drug-resistant cancer stem cells (CSCs). Herein, a pH-sensitive nanoparticle with simultaneous encapsulation of curcumin and doxorubicin (CURDOX-NPs) was prepared by using monomethoxy (polyethylene glycol)-b-P (D,L-lactic-co-glycolic acid)-b-P (L-glutamic acid) polymer to simultaneously target the differentiated tumor cells and CSCs. CURDOX-NPs had a mean diameter of 107.5 nm and zeta potential of -13.7 mV, determined by DLS. Drug-loading efficiency for curcumin and doxorubicin was reaching to 80.30% and 96.2%, respectively. Moreover, a cascade sustained-release profiles with the faster release of CUR followed by a slower release of DOX was observed in normal pH7.4 condition. Moreover, a pH-sensitive release profile for each cargo was seen in pH5.0 condition. The anti-tumour effect of CURDOX-NPs on CSCs-enriching MCF-7/ADR mammospheres was confirmed by in vitro. Moreover, a significant regression of tumour growth after treatment with CURDOX-NPs was also observed in Xenograft mice model. The percentage of CSCs in tumour significantly decreased from 39.9% in control group to 6.82% after treatment with CURDOX-NPs. The combinational delivery of CUR and DOX may a potentially useful therapeutic strategy for refractory breast cancer.
- Subjects :
- Animals
Capsules
Cell Proliferation drug effects
Drug Carriers chemistry
Drug Liberation
Humans
Hydrogen-Ion Concentration
MCF-7 Cells
Mice
Particle Size
Polyglutamic Acid chemistry
Xenograft Model Antitumor Assays
Breast Neoplasms pathology
Curcumin chemistry
Curcumin pharmacology
Doxorubicin chemistry
Doxorubicin pharmacology
Nanoparticles chemistry
Polyesters chemistry
Polyethylene Glycols chemistry
Polyglutamic Acid analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 2169-141X
- Volume :
- 46
- Issue :
- sup1
- Database :
- MEDLINE
- Journal :
- Artificial cells, nanomedicine, and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 29301415
- Full Text :
- https://doi.org/10.1080/21691401.2017.1423495