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Proteome of Bovine Mitochondria and Rod Outer Segment Disks: Commonalities and Differences.
- Source :
-
Journal of proteome research [J Proteome Res] 2018 Feb 02; Vol. 17 (2), pp. 918-925. Date of Electronic Publication: 2018 Jan 18. - Publication Year :
- 2018
-
Abstract
- The retinal rod outer segment (OS) is a stack of disks surrounded by the plasma membrane, housing proteins related to phototransduction, as well as mitochondrial proteins involved in oxidative phosphorylation (OxPhos). This prompted us to compare the proteome of bovine OS disks and mitochondria to assess the significant top gene signatures of each sample. The two proteomes, obtained by LTQ-Orbitrap Velos mass spectrometry, were compared by statistical analyses. In total, 4139 proteins were identified, 2045 of which overlapping in the two sets. Nonhierarchical Spearman's correlogram revealed that the groups were clearly discriminated. Partial least square discriminant plus support vector machine analysis identified the major discriminative proteins, implied in phototransduction and lipid metabolism, respectively. Gene Ontology analysis identified top gene signatures of the disk proteome, enriched in vesiculation, glycolysis, and OxPhos proteins. The tricarboxylic acid cycle and the electron transport proteins were similarly enriched in the two samples, but the latter was up regulated in disks. Data suggest that the mitochondrial OxPhos proteins may represent a true OS proteome component, outside the mitochondrion. This knowledge may help the scientific community in the further studies of retinal physiology and pathology.
- Subjects :
- Animals
Cattle
Chromatography, Liquid
Citric Acid Cycle genetics
Eye Proteins genetics
Eye Proteins metabolism
Gene Ontology
Glycolysis genetics
Least-Squares Analysis
Light Signal Transduction
Lipid Metabolism genetics
Microscopy, Electron, Transmission
Mitochondria metabolism
Mitochondria ultrastructure
Mitochondrial Proteins genetics
Mitochondrial Proteins metabolism
Molecular Sequence Annotation
Oxidative Phosphorylation
Proteome genetics
Proteome metabolism
Rod Cell Outer Segment ultrastructure
Support Vector Machine
Tandem Mass Spectrometry
Eye Proteins isolation & purification
Mitochondria genetics
Mitochondrial Proteins isolation & purification
Proteome isolation & purification
Rod Cell Outer Segment metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1535-3907
- Volume :
- 17
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of proteome research
- Publication Type :
- Academic Journal
- Accession number :
- 29299929
- Full Text :
- https://doi.org/10.1021/acs.jproteome.7b00741