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Crustacean cardioactive peptides: Expression, localization, structure, and a possible involvement in regulation of egg-laying in the cuttlefish Sepia officinalis.
- Source :
-
General and comparative endocrinology [Gen Comp Endocrinol] 2018 May 01; Vol. 260, pp. 67-79. Date of Electronic Publication: 2017 Dec 24. - Publication Year :
- 2018
-
Abstract
- The cuttlefish (Sepia officinalis) is a cephalopod mollusk distributed on the western European coast, in the West African Ocean and in the Mediterranean Sea. On the Normandy coast (France), cuttlefish is a target species of professional fishermen, so its reproduction strategy is of particular interest in the context of stock management. Egg-laying, which is coastal, is controlled by several types of regulators among which neuropeptides. The cuttlefish neuropeptidome was recently identified by Zatylny-Gaudin et al. (2016). Among the 38 neuropeptide families identified, some were significantly overexpressed in egg-laying females as compared to mature males. This study is focused on crustacean cardioactive peptides (CCAPs), a highly expressed neuropeptide family strongly suspected of being involved in the control of egg-laying. We investigated the functional and structural characterization and tissue mapping of CCAPs, as well as the expression patterns of their receptors. CCAPs appeared to be involved in oocyte transport through the oviduct and in mechanical secretion of capsular products. Immunocytochemistry revealed that the neuropeptides were localized throughout the central nervous system (CNS) and in the nerve endings of the glands involved in egg-capsule synthesis and secretion, i.e. the oviduct gland and the main nidamental glands. The CCAP receptor was expressed in these glands and in the subesophageal mass of the CNS. Multiple sequence alignments revealed a high level of conservation of CCAP protein precursors in Sepia officinalis and Loligo pealei, two cephalopod decapods. Primary sequences of CCAPs from the two species were fully conserved, and cryptic peptides detected in the nerve endings were also partially conserved, suggesting biological activity that remains unknown for the time being.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Central Nervous System metabolism
Decapodiformes genetics
Decapodiformes growth & development
Decapodiformes metabolism
Female
Gene Expression Regulation, Developmental
Male
Oocytes metabolism
Oviducts metabolism
Oviposition genetics
Reproduction genetics
Sepia growth & development
Sepia physiology
Sequence Alignment
Tissue Distribution
Neuropeptides chemistry
Neuropeptides genetics
Neuropeptides metabolism
Oviparity genetics
Sepia genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1095-6840
- Volume :
- 260
- Database :
- MEDLINE
- Journal :
- General and comparative endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 29278693
- Full Text :
- https://doi.org/10.1016/j.ygcen.2017.12.009