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Proteomics and bioinformatics analysis of altered protein expression in the placental villous tissue from early recurrent miscarriage patients.
- Source :
-
Placenta [Placenta] 2018 Jan; Vol. 61, pp. 1-10. Date of Electronic Publication: 2017 Nov 04. - Publication Year :
- 2018
-
Abstract
- Introduction: Recurrent miscarriage (RM) affects 5% of women, it has an adverse emotional impact on women. Because of the complexities of early development, the mechanism of recurrent miscarriage is still unclear. We hypothesized that abnormal placenta leads to early recurrent miscarriage (ERM). The aim of this study was to identify ERM associated factors in human placenta villous tissue using proteomics. Investigation of these differences in protein expression in parallel profiling is essential to understand the comprehensive pathophysiological mechanism underlying recurrent miscarriage (RM).<br />Methods: To gain more insight into mechanisms of recurrent miscarriage (RM), a comparative proteome profile of the human placenta villous tissue in normal and RM pregnancies was analyzed using iTRAQ technology and bioinformatics analysis used by Ingenuity Pathway Analysis (IPA) software.<br />Results: In this study, we employed an iTRAQ based proteomics analysis of four placental villous tissues from patients with early recurrent miscarriage (ERM) and four from normal pregnant women. Finally, we identified 2805 proteins and 79,998 peptides between patients with RM and normal matched group. Further analysis identified 314 differentially expressed proteins in placental villous tissue (≥1.3-fold, Student's t-test, p < 0.05); 209 proteins showed the increased expression while 105 proteins showed decreased expression. These 314 proteins were analyzed by Ingenuity Pathway Analysis (IPA) and were found to play important roles in the growth of embryo. Furthermore, network analysis show that Angiotensinogen (AGT), MAPK14 and Prothrombin (F2) are core factors in early embryonic development. We used another 8 independent samples (4 cases and 4 controls) to cross validation of the proteomic data.<br />Discussion: This study has identified several proteins that are associated with early development, these results may supply new insight into mechanisms behind recurrent miscarriage.<br /> (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Subjects :
- Abortion, Habitual enzymology
Adult
Angiotensinogen genetics
China
Chorionic Villi enzymology
Cluster Analysis
Computational Biology
Embryonic Development
Female
Gene Expression Profiling
Humans
Mitogen-Activated Protein Kinase 14 genetics
Placenta enzymology
Placenta metabolism
Placentation
Pregnancy
Pregnancy Trimester, First
Proteomics methods
Prothrombin genetics
Software
Abortion, Habitual metabolism
Angiotensinogen metabolism
Chorionic Villi metabolism
Gene Expression Regulation, Developmental
Gene Regulatory Networks
Mitogen-Activated Protein Kinase 14 metabolism
Prothrombin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1532-3102
- Volume :
- 61
- Database :
- MEDLINE
- Journal :
- Placenta
- Publication Type :
- Academic Journal
- Accession number :
- 29277264
- Full Text :
- https://doi.org/10.1016/j.placenta.2017.11.001