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Generation of a whole-brain atlas for the cholinergic system and mesoscopic projectome analysis of basal forebrain cholinergic neurons.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2018 Jan 09; Vol. 115 (2), pp. 415-420. Date of Electronic Publication: 2017 Dec 19. - Publication Year :
- 2018
-
Abstract
- The cholinergic system in the brain plays crucial roles in regulating sensory and motor functions as well as cognitive behaviors by modulating neuronal activity. Understanding the organization of the cholinergic system requires a complete map of cholinergic neurons and their axon arborizations throughout the entire brain at the level of single neurons. Here, we report a comprehensive whole-brain atlas of the cholinergic system originating from various cortical and subcortical regions of the mouse brain. Using genetically labeled cholinergic neurons together with whole-brain reconstruction of optical images at 2-μm resolution, we obtained quantification of the number and soma volume of cholinergic neurons in 22 brain areas. Furthermore, by reconstructing the complete axonal arbors of fluorescently labeled single neurons from a subregion of the basal forebrain at 1-μm resolution, we found that their projections to the forebrain and midbrain showed neuronal subgroups with distinct projection specificity and diverse arbor distribution within the same projection area. These results suggest the existence of distinct subtypes of cholinergic neurons that serve different regulatory functions in the brain and illustrate the usefulness of complete reconstruction of neuronal distribution and axon projections at the mesoscopic level.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Animals
Basal Forebrain anatomy & histology
Basal Forebrain diagnostic imaging
Brain anatomy & histology
Brain diagnostic imaging
Cell Count
Cerebral Cortex anatomy & histology
Cerebral Cortex diagnostic imaging
Mesencephalon anatomy & histology
Mesencephalon cytology
Mesencephalon diagnostic imaging
Mice
Models, Anatomic
Basal Forebrain cytology
Brain cytology
Cerebral Cortex cytology
Cholinergic Neurons cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1091-6490
- Volume :
- 115
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 29259118
- Full Text :
- https://doi.org/10.1073/pnas.1703601115