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Lung Vascular Remodeling, Cardiac Hypertrophy, and Inflammatory Cytokines in SHIVnef-Infected Macaques.
- Source :
-
Viral immunology [Viral Immunol] 2018 Apr; Vol. 31 (3), pp. 206-222. Date of Electronic Publication: 2017 Dec 19. - Publication Year :
- 2018
-
Abstract
- Fatal pulmonary arterial hypertension (PAH) affects HIV-infected individuals at significantly higher frequencies. We previously showed plexiform-like lesions characterized by recanalized lumenal obliteration, intimal disruption, medial hypertrophy, and thrombosis consistent with PAH in rhesus macaques infected with chimeric SHIVnef but not with the parental SIVmac239, suggesting that Nef is implicated in the pathophysiology of HIV-PAH. However, the current literature on non-human primates as animal models for SIV(HIV)-associated pulmonary disease reports the ultimate pathogenic pulmonary outcomes of the research efforts; however, the variability and features in the actual disease progression remain poorly described, particularly when using different viral sources for infection. We analyzed lung histopathology, performed immunophenotyping of cells in plexogenic lesions pathognomonic of PAH, and measured cardiac hypertrophy biomarkers and cytokine expression in plasma and lung of juvenile SHIVnef-infected macaques. Here, we report significant hematopathologies, changes in cardiac biomarkers consistent with ventricular hypertrophy, significantly increased levels of interleukin-12 and GM-CSF and significantly decreased sCD40 L, CCL-2, and CXCL-1 in plasma of the SHIVnef group. Pathway analysis of inflammatory gene expression predicted activation of NF-κB transcription factor RelB and inhibition of bone morphogenetic protein type-2 in the setting of SHIVnef infection. Our findings highlight the utility of SHIVnef-infected macaques as suitable models of HIV-associated pulmonary vascular remodeling as pathogenetic changes are concordant with features of idiopathic, familial, scleroderma, and HIV-PAH.
- Subjects :
- Animals
Gene Expression Profiling
HIV genetics
HIV growth & development
Histocytochemistry
Immunophenotyping
Male
Plasma chemistry
Simian Acquired Immunodeficiency Syndrome virology
Simian Immunodeficiency Virus genetics
Simian Immunodeficiency Virus growth & development
Cardiomegaly pathology
Cytokines analysis
Hypertension, Pulmonary pathology
Lung pathology
Macaca mulatta
Simian Acquired Immunodeficiency Syndrome complications
Vascular Remodeling
Subjects
Details
- Language :
- English
- ISSN :
- 1557-8976
- Volume :
- 31
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Viral immunology
- Publication Type :
- Academic Journal
- Accession number :
- 29256819
- Full Text :
- https://doi.org/10.1089/vim.2017.0051