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The role of gut in type 2 diabetes mellitus during whole body gamma irradiation in high-fat diet Wistar rats.
- Source :
-
International journal of radiation biology [Int J Radiat Biol] 2018 Feb; Vol. 94 (2), pp. 137-149. Date of Electronic Publication: 2018 Jan 03. - Publication Year :
- 2018
-
Abstract
- Purpose: The effects of a low rate (100 mGy/min) fractionated whole body gamma irradiation (FWBGI) at different doses were assessed using a real-time PCR technique on the expression of some target genes implicated in the development of type 2 diabetes mellitus in high-fat diet (HFD) Wistar rats.<br />Method: HFD Wistar rats were exposed to different doses (12, 24 and 48 Gy) divided into 24 fractions (three times a week for two months), thus, the daily doses were 0.5, 1, 2 Gy, respectively. Total RNA was extracted and the expression of target genes was measured in the four intestinal segments (duodenum, jejunum, ileum and colon).<br />Results: The pre-diabetic state already induced by HFD was found to be improved by irradiation exposure. This irradiation effect occurs mainly via altered anti-diabetic gene expressions (mRNA and protein levels) of the incretin glucagon-like peptide-1 (GLP-1) overall bowel segments except the colon which has its own specific response to irradiation exposure by the induction of the insulin receptor substrate 4 (IRS-4) and the uncoupling protein 3 (UCP3).<br />Conclusions: Results could be of great importance suggesting for the first time, a protective role for FWBGI on HFD animal models by increasing GLP-1 and UCP3 levels.
- Subjects :
- Animals
Diabetes Mellitus, Experimental metabolism
Diet, High-Fat
Dose-Response Relationship, Radiation
Glucagon-Like Peptide 1 metabolism
Glucose Tolerance Test
Insulin Receptor Substrate Proteins metabolism
Male
RNA, Messenger metabolism
Rats
Rats, Wistar
Real-Time Polymerase Chain Reaction
Uncoupling Protein 3 metabolism
Diabetes Mellitus, Type 2 metabolism
Gene Expression Regulation
Whole-Body Irradiation
Subjects
Details
- Language :
- English
- ISSN :
- 1362-3095
- Volume :
- 94
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of radiation biology
- Publication Type :
- Academic Journal
- Accession number :
- 29252073
- Full Text :
- https://doi.org/10.1080/09553002.2018.1419300