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RNAs coordinate nuclear envelope assembly and DNA replication through ELYS recruitment to chromatin.
- Source :
-
Nature communications [Nat Commun] 2017 Dec 14; Vol. 8 (1), pp. 2130. Date of Electronic Publication: 2017 Dec 14. - Publication Year :
- 2017
-
Abstract
- Upon fertilisation, the sperm pronucleus acquires the competence to replicate the genome through a cascade of events that link chromatin remodelling to nuclear envelope formation. The factors involved have been partially identified and are poorly characterised. Here, using Xenopus laevis egg extracts we show that RNAs are required for proper nuclear envelope assembly following sperm DNA decondensation. Although chromatin remodelling and pre-replication complex formation occur normally, RNA-depleted extracts show a defect in pre-RC activation. The nuclear processes affected by RNA-depletion included ELYS recruitment, which accounts for the deficiency in nuclear pore complex assembly. This results in failure in chromatin relaxation as well as in the import and proper nuclear concentration of the S-phase kinases necessary for DNA replication activation. Our results highlight a translation-independent RNA function necessary for the parental genome progression towards the early embryonic cell cycle programme.
- Subjects :
- Animals
Cell Cycle drug effects
Cell Cycle genetics
Cell Extracts chemistry
Cell Extracts pharmacology
Cell Nucleus genetics
Cell Nucleus metabolism
Chromatin genetics
Chromatin metabolism
DNA genetics
DNA metabolism
DNA-Binding Proteins genetics
Male
Nuclear Envelope genetics
Nuclear Pore genetics
Nuclear Pore metabolism
Ovum cytology
Ovum metabolism
RNA genetics
Spermatozoa metabolism
Transcription Factors genetics
Xenopus Proteins genetics
Xenopus laevis
DNA Replication
DNA-Binding Proteins metabolism
Nuclear Envelope metabolism
RNA metabolism
Transcription Factors metabolism
Xenopus Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 29242643
- Full Text :
- https://doi.org/10.1038/s41467-017-02180-1