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Lipid-Controlled Stabilization of Charge-Separated States (P + Q B - ) and Photocurrent Generation Activity of a Light-Harvesting-Reaction Center Core Complex (LH1-RC) from Rhodopseudomonas palustris.
- Source :
-
The journal of physical chemistry. B [J Phys Chem B] 2018 Jan 25; Vol. 122 (3), pp. 1066-1080. Date of Electronic Publication: 2018 Jan 04. - Publication Year :
- 2018
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Abstract
- The photosynthetic light-harvesting-reaction center core complex (LH1-RC) is a natural excitonic and photovoltaic device embedded in a lipid membrane. In order to apply LH1-RCs as a biohybrid energy-producing material, some important issues must be addressed, including how to make LH1-RCs function as efficiently as possible. In addition, they should be characterized to evaluate how many active LH1-RCs efficiently work in artificial systems. We report here that an anionic phospholipid, phosphatidylglycerol (PG), stabilizes the charge-separated state (a photooxidized electron donor and reduced quinone pair, P <superscript>+</superscript> Q <subscript>B</subscript> <superscript>-</superscript> ) of LH1-RC (from Rhodopseudomonas palustris) and enhances its activity in photocurrent generation. Steady-state fluorometric analysis demonstrated that PG enhances the formation of the P <superscript>+</superscript> Q <subscript>B</subscript> <superscript>-</superscript> state at lower irradiances. The photocurrent generation activity was analyzed via Michaelis-Menten kinetics, revealing that 38% of LH1-RCs reconstituted into the PG membrane generated photocurrent at a turnover frequency of 46 s <superscript>-1</superscript> . PG molecules, which interact with LH1-RC in vivo, play the role of an active effector component for LH1-RC to enhance its function in the biohybrid system.
Details
- Language :
- English
- ISSN :
- 1520-5207
- Volume :
- 122
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The journal of physical chemistry. B
- Publication Type :
- Academic Journal
- Accession number :
- 29236490
- Full Text :
- https://doi.org/10.1021/acs.jpcb.7b09973