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Amine oxidases as important agents of pathological processes of rhabdomyolysis in rats.
- Source :
-
Ukrainian biochemical journal [Ukr Biochem J] 2016 Jan-Feb; Vol. 88 (1), pp. 79-87. - Publication Year :
- 2016
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Abstract
- In this study we have tested an idea on the important role of amine oxidases (semicarbazide-sensitive amine oxidase, diamine oxidase, polyamine oxidase) as an additional source of oxidative/carbonyl stress under glycerol-induced rhabdomyolysis, since the enhanced formation of reactive oxygen species and reactive carbonyl species in a variety of tissues is linked to various diseases. In our experiments we used the sensitive fluorescent method devised for estimation of amine oxidases activity in the rat kidney and thymus as targeted organs under rhabdomyolysis. We have found in vivo the multiple rises in activity of semicarbazide-sensitive amine oxidase, diamine oxidase, polyamine oxidase (2-4.5 times) in the corresponding cell fractions, whole cells or their lysates at the 3-6th day after glycerol injection. Aberrant antioxidant activities depended on rhabdomyolysis stage and had organ specificity. Additional treatment of animals with metal chelator ‘Unithiol’ adjusted only the activity of antioxidant enzymes but not amine oxidases in both organs. Furthermore the in vitro experiment showed that Fenton reaction (hydrogen peroxide in the presence of iron) products alone had no effect on semicarbazide-sensitive amine oxidase activity in rat liver cell fraction whereas supplementation with methylglyoxal resulted in its significant 2.5-fold enhancement. Combined action of the both agents had additive effect on semicarbazide-sensitive amine oxidase activity. We can assume that biogenic amine and polyamine catabolism by amine oxidases is upregulated by oxidative and carbonyl stress factors directly under rhabdomyolysis progression, and the increase in catabolic products concentration contributes to tissue damage in glycerol-induced acute renal failure and apoptosis stimulation in thymus.
- Subjects :
- Animals
Chelating Agents pharmacology
Glycerol
Hepatocytes drug effects
Hepatocytes enzymology
Hepatocytes pathology
Hydrogen Peroxide antagonists & inhibitors
Hydrogen Peroxide pharmacology
Kidney drug effects
Kidney enzymology
Kidney pathology
Liver drug effects
Liver enzymology
Liver pathology
Male
Organ Specificity
Oxidation-Reduction
Protein Carbonylation
Pyruvaldehyde antagonists & inhibitors
Pyruvaldehyde pharmacology
Rats
Rats, Wistar
Rhabdomyolysis chemically induced
Rhabdomyolysis drug therapy
Rhabdomyolysis pathology
Semicarbazides antagonists & inhibitors
Semicarbazides pharmacology
Thymus Gland drug effects
Thymus Gland enzymology
Thymus Gland pathology
Unithiol pharmacology
Polyamine Oxidase
Amine Oxidase (Copper-Containing) metabolism
Monoamine Oxidase metabolism
Oxidoreductases Acting on CH-NH Group Donors metabolism
Reactive Oxygen Species metabolism
Rhabdomyolysis enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 2409-4943
- Volume :
- 88
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Ukrainian biochemical journal
- Publication Type :
- Academic Journal
- Accession number :
- 29227084
- Full Text :
- https://doi.org/10.15407/ubj88.01.079