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Preventive effect of anti-VacA egg yolk immunoglobulin (IgY) on Helicobacter pylori-infected mice.
- Source :
-
Vaccine [Vaccine] 2018 Jan 08; Vol. 36 (3), pp. 371-380. Date of Electronic Publication: 2017 Dec 06. - Publication Year :
- 2018
-
Abstract
- Background: Helicobacter pylori, a gram-negative bacterium, is the causative agent of gastric disorders and gastric cancer in the human stomach. Vacuolating cytotoxin A (VacA) is among the multi-effect protein toxins released by H. pylori that enables its persistence in the human stomach.<br />Methods: To evaluate the effect of anti-VacA egg yolk immunoglobulin (anti-VacA IgY) on H. pylori infection, a highly specific anti-VacA IgY was produced from egg yolks of hens immunized with a mixture of two purified recombinant VacAs. Female C57BL/6 mice were supplemented anti-VacA IgY daily with drinking water for 2 weeks before and 4 weeks after H. pylori ATCC 43504 inoculation. Anti-VacA IgY recognized both native and denatured structures of VacA by enzyme-linked immunosorbent assay and immunoblotting analyses, respectively.<br />Results: Oral administration of anti-VacA IgYs significantly (p < .05) reduced the serum levels of anti-H. pylori antibodies compared to those in the H. pylori-infected, untreated group. The reduction in the immune response was accompanied by a significant (p < .05) decrease in eosinophilic infiltration of the stomach in anti-VacA IgY treated group compared to other groups. Concomitantly, H. pylori-induced histological changes and H. pylori antigen-positivity in gastric tissues were decreased significantly (p < .05) in anti-VacA IgY treated group similar to the control group.<br />Conclusions: Oral administration of anti-VacA IgY is correlated with a protective effect against H. pylori colonization and induced histological changes in gastric tissues. Our experimental study has proved that it is expected to be a new drug candidate of Hp infection by further study.<br /> (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Subjects :
- Administration, Oral
Animals
Disease Models, Animal
Female
Mice, Inbred C57BL
Treatment Outcome
Antibodies, Bacterial administration & dosage
Bacterial Proteins antagonists & inhibitors
Helicobacter Infections prevention & control
Helicobacter pylori immunology
Immunoglobulins administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2518
- Volume :
- 36
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 29223485
- Full Text :
- https://doi.org/10.1016/j.vaccine.2017.11.082