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Molecular characterization of virulence and antimicrobial resistance profile of Shigella species isolated from children with moderate to severe diarrhea in northeastern Brazil.
- Source :
-
Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2018 Mar; Vol. 90 (3), pp. 198-205. Date of Electronic Publication: 2017 Nov 10. - Publication Year :
- 2018
-
Abstract
- Molecular characterization of virulence and antimicrobial resistance profiles were determined for Shigella species isolated from children with diarrhea in Fortaleza, Brazil. Fecal specimens were collected along with socioeconomic and clinical data from children with moderate to severe diarrhea requiring emergency care. Shigella spp. were isolated by standard microbiological techniques, and we developed 4 multiplex polymerase chain reaction assays to detect 16 virulence-related genes (VRGs). Antimicrobial susceptibility tests were performed using disk diffusion assays. S. flexneri and S. sonnei were the predominant serogroups. S. flexneri was associated with low monthly incomes; more severe disease; higher number of VRGs; and presence of pic, set, and sepA genes. The SepA gene was associated with more intense abdominal pain. S. flexneri was correlated with resistance to ampicillin and chloramphenicol, whereas S. sonnei was associated with resistance to azithromycin. Strains harboring higher numbers of VRGs were associated with resistance to more antimicrobials. We highlight the correlation between presence of S. flexneri and sepA, and increased virulence and suggest a link to socioeconomic change in northeastern Brazil. Additionally, antimicrobial resistance was associated with serogroup specificity in Shigella spp. and increased bacterial VRGs.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Subjects :
- Ampicillin pharmacology
Azithromycin pharmacology
Bacterial Proteins genetics
Brazil
Chloramphenicol pharmacology
Disk Diffusion Antimicrobial Tests
Drug Resistance, Bacterial
Dysentery, Bacillary drug therapy
Humans
Multiplex Polymerase Chain Reaction methods
Serine Proteases genetics
Shigella flexneri drug effects
Shigella flexneri isolation & purification
Shigella sonnei drug effects
Shigella sonnei isolation & purification
Virulence genetics
Anti-Bacterial Agents pharmacology
Dysentery, Bacillary microbiology
Shigella flexneri genetics
Shigella flexneri pathogenicity
Shigella sonnei genetics
Shigella sonnei pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0070
- Volume :
- 90
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Diagnostic microbiology and infectious disease
- Publication Type :
- Academic Journal
- Accession number :
- 29217418
- Full Text :
- https://doi.org/10.1016/j.diagmicrobio.2017.11.002