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Dynamic changes in transcriptome and cell wall composition underlying brassinosteroid-mediated lignification of switchgrass suspension cells.

Authors :
Rao X
Shen H
Pattathil S
Hahn MG
Gelineo-Albersheim I
Mohnen D
Pu Y
Ragauskas AJ
Chen X
Chen F
Dixon RA
Source :
Biotechnology for biofuels [Biotechnol Biofuels] 2017 Nov 30; Vol. 10, pp. 266. Date of Electronic Publication: 2017 Nov 30 (Print Publication: 2017).
Publication Year :
2017

Abstract

Background: Plant cell walls contribute the majority of plant biomass that can be used to produce transportation fuels. However, the complexity and variability in composition and structure of cell walls, particularly the presence of lignin, negatively impacts their deconstruction for bioenergy. Metabolic and genetic changes associated with secondary wall development in the biofuel crop switchgrass ( Panicum virgatum ) have yet to be reported.<br />Results: Our previous studies have established a cell suspension system for switchgrass, in which cell wall lignification can be induced by application of brassinolide (BL). We have now collected cell wall composition and microarray-based transcriptome profiles for BL-induced and non-induced suspension cultures to provide an overview of the dynamic changes in transcriptional reprogramming during BL-induced cell wall modification. From this analysis, we have identified changes in candidate genes involved in cell wall precursor synthesis, cellulose, hemicellulose, and pectin formation and ester-linkage generation. We have also identified a large number of transcription factors with expression correlated with lignin biosynthesis genes, among which are candidates for control of syringyl (S) lignin accumulation.<br />Conclusion: Together, this work provides an overview of the dynamic compositional changes during brassinosteroid-induced cell wall remodeling, and identifies candidate genes for future plant genetic engineering to overcome cell wall recalcitrance.

Details

Language :
English
ISSN :
1754-6834
Volume :
10
Database :
MEDLINE
Journal :
Biotechnology for biofuels
Publication Type :
Academic Journal
Accession number :
29213317
Full Text :
https://doi.org/10.1186/s13068-017-0954-2