In Vitro Antibacterial Activity of Pomegranate Juice and Peel Extracts on Cariogenic Bacteria.

Aim: To evaluate the antimicrobial activity of hydroalcoholic extracts of pomegranate ( Punica granatum L.) peel and juice, against the microorganisms considered the main etiologic agents of dental caries.
Methods: The values of the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined against Streptococcus mutans Clarke ATCC® 25175™ strain and Rothia dentocariosa clinical isolate.
Results: Peel extracts inhibit effectively the growth and survival of S. mutans ATCC 25175 strain and R. dentocariosa clinical isolate with MIC and MBC values of 10  μ g/ μ l and 15  μ g/ μ l, respectively. Furthermore, the pomegranate juice extract showed high inhibitory activity against S. mutans ATCC 25175 strain with a MIC value of 25  μ g/ μ l and a MBC value of 40  μ g/ μ l, whereas, against R. dentocariosa , it has displayed a moderate inhibitory activity, with MIC and MBC values of 20  μ g/ μ l and 140  μ g/ μ l, respectively.
Conclusions: In vitro microbiological tests demonstrate that the hydroalcoholic extracts of pomegranate juice and peel are able to contrast the main cariogenic bacteria involved in tooth decay. Although being preliminary data, our results suggest that pomegranate polyphenolic compounds could represent a good adjuvant for the prevention and treatment of dental caries.