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Characterization of a New Ezakiella Isolated from the Human Vagina: Genome Sequence and Description of Ezakiella massiliensis sp. nov.
- Source :
-
Current microbiology [Curr Microbiol] 2018 Apr; Vol. 75 (4), pp. 456-463. Date of Electronic Publication: 2017 Nov 29. - Publication Year :
- 2018
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Abstract
- The study of the vaginal microbiota using the "culturomics concept" allowed us to isolate, from the vaginal swab of an asymptomatic 20-year-old woman who had sexual relations with another woman with bacterial vaginosis, an unknown Gram-positive anaerobic coccus-shaped bacterium that was designated strain Marseille-P2951 <superscript>T</superscript> and characterized using taxono-genomics. Strain Marseille-P2951 <superscript>T</superscript> is non-motile and non-spore forming and exhibits catalase and oxidase activities. Its 16S rRNA gene-based identification showed 98.5% identity with Ezakiella peruensis, the phylogenetically closest species. The major fatty acids are C18:1n9 (58%) and C16:0 (22%). With a 1,741,785 bp length, the G+C content of the genome is 36.69%. Of a total of 1657 genes, 1606 are protein-coding genes and 51 RNAs. Also, 1123 genes are assigned a putative function and 127 are ORFans. Phenotypic, phylogenetic, and genomics analyses revealed that strain Marseille-P2951 <superscript>T</superscript> (=CSUR P2951 =DSM 103122) is distinct and represents a new species of the genus Ezakiella, for which the name Ezakiella massiliensis sp. nov. is proposed.
Details
- Language :
- English
- ISSN :
- 1432-0991
- Volume :
- 75
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Current microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 29188320
- Full Text :
- https://doi.org/10.1007/s00284-017-1402-z