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Mechanism of bioactive molecular extraction from mineralized dentin by calcium hydroxide and tricalcium silicate cement.
- Source :
-
Dental materials : official publication of the Academy of Dental Materials [Dent Mater] 2018 Feb; Vol. 34 (2), pp. 317-330. Date of Electronic Publication: 2017 Nov 24. - Publication Year :
- 2018
-
Abstract
- Objectives: The objective of the present study was to elucidate the mechanism of bioactive molecule extraction from mineralized dentin by calcium hydroxide (Ca(OH) <subscript>2</subscript> ) and tricalcium silicate cements (TSC).<br />Methods and Results: Transmission electron microscopy was used to provide evidence for collagen degradation in dentin surfaces covered with Ca(OH) <subscript>2</subscript> or a set, hydrated TSC for 1-3 months. A one micron thick collagen degradation zone was observed on the dentin surface. Fourier transform-infrared spectroscopy was used to identify increases in apatite/collagen ratio in dentin exposed to Ca(OH) <subscript>2</subscript> . Using three-point bending, dentin exposed to Ca(OH) <subscript>2</subscript> exhibited significant reduction in flexural strength. Using size exclusion chromatography, it was found that the small size of the hydroxyl ions derived from Ca(OH) <subscript>2</subscript> enabled those ions to infiltrate the intrafibrillar compartment of mineralized collagen and degrade the collagen fibrils without affecting the apatite minerals. Using ELISA, TGF-β1 was found to be extracted from dentin covered with Ca(OH) <subscript>2</subscript> for 3 months. Unlike acids that dissolve the mineral component of dentin to release bioactive molecules, alkaline materials such as Ca(OH) <subscript>2</subscript> or TSC released growth factors such as TGF-β1 via collagen degradation.<br />Significance: The bioactive molecule extraction capacities of Ca(OH) <subscript>2</subscript> and TSC render these dental materials excellent for pulp capping and endodontic regeneration. These highly desirable properties, however, appear to be intertwined with the untoward effect of degradation of the collagen matrix within mineralized dentin, resulting in reduced flexural strength.<br /> (Copyright © 2017 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.)
- Subjects :
- Apatites chemistry
Dental Pulp Capping
Dentin chemistry
Humans
In Vitro Techniques
Materials Testing
Microscopy, Electron, Transmission
Spectroscopy, Fourier Transform Infrared
Calcium Compounds chemistry
Calcium Hydroxide chemistry
Collagen drug effects
Collagen metabolism
Dental Cements chemistry
Dentin drug effects
Silicates chemistry
Transforming Growth Factor beta1 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0097
- Volume :
- 34
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Dental materials : official publication of the Academy of Dental Materials
- Publication Type :
- Academic Journal
- Accession number :
- 29179973
- Full Text :
- https://doi.org/10.1016/j.dental.2017.11.010