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In vitro inoculation of Aeromonas hydrophila and Pseudomonas fluorescens in cryopreserved silver barb (Barbodes gonionotus) milt: Effect on fertilization capacity and transmission potential to embryos.
- Source :
-
Theriogenology [Theriogenology] 2018 Mar 01; Vol. 108, pp. 1-6. Date of Electronic Publication: 2017 Nov 16. - Publication Year :
- 2018
-
Abstract
- Purposive use of cryopreserved sperm contaminated with pathogenic agents has increased the risk of spreading of fish diseases. The objective of this study was to investigate the effects of A. hydrophila subsp. hydrophila and P. fluorescens inoculations into cryostored milt on fertilization capacity and transmission potential to embryos of silver barb (Barbodes gonionotus) with or without 0.25% penicillin-streptomycin (PS) administration. The experiment comprised six treatments: addition of milt into T1) extender only, T2) extender with 0.25% PS, T3) extender with A. hydrophila subsp. hydrophila (BG19), T4) extender with A. hydrophila subsp. hydrophila (BG19) and 0.25% PS, T5) extender with P. fluorescens (BG20) and T6) extender with P. fluorescens (BG20) and 0.25% PS. Milt were loaded into 0.25-mL straws and cryostored in the controlled-rate programmable freezer. After a cryostorage for 28 d, post-thawed sperm were evaluated for the fertilization capacity and risk of pathogen transmission to embryos. Inoculation of A. hydrophila subsp. hydrophila and P. fluorescens into extended milt (T3 and T5) caused a reduction (P < 0.05) in fertilization capacity of cryopreserved sperm. Cryopreserved sperm inoculated with the two pathogenic bacteria and 0.25% PS (T4 and T6) did not fertilize the eggs. The two pathogenic bacteria could be transmitted into embryos after artificial insemination of eggs with bacterial-inoculated cryopreserved sperm, suggesting that the risks of disease transmission via cryopreserved fish sperm would exist. This is the first study reporting pathogenic bacterial transmission on in vitro fish embryos through artificial insemination of cryopreserved sperm.<br /> (Copyright © 2017. Published by Elsevier Inc.)
- Subjects :
- Animals
Cyprinidae microbiology
Embryo, Nonmammalian microbiology
Fish Diseases microbiology
Fish Diseases transmission
Gram-Negative Bacterial Infections transmission
Male
Semen microbiology
Semen Analysis
Semen Preservation
Sperm Motility
Spermatozoa
Aeromonas hydrophila physiology
Cryopreservation veterinary
Cyprinidae embryology
Gram-Negative Bacterial Infections veterinary
Pseudomonas fluorescens physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3231
- Volume :
- 108
- Database :
- MEDLINE
- Journal :
- Theriogenology
- Publication Type :
- Academic Journal
- Accession number :
- 29175681
- Full Text :
- https://doi.org/10.1016/j.theriogenology.2017.11.009