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Molecular pathology of total knee arthroplasty instability defined by RNA-seq.

Authors :
Lewallen EA
Salib CG
Trousdale WH
Berry CE
Hanssen GM
Robin JX
Tibbo ME
Viste A
Reina N
Morrey ME
Sanchez-Sotelo J
Hanssen AD
Berry DJ
van Wijnen AJ
Abdel MP
Source :
Genomics [Genomics] 2018 Sep; Vol. 110 (5), pp. 247-256. Date of Electronic Publication: 2017 Nov 22.
Publication Year :
2018

Abstract

Total knee arthroplasty (TKA) is a durable and reliable procedure to alleviate pain and improve joint function. However, failures related to flexion instability sometimes occur. The goal of this study was to define biological differences between tissues from patients with and without flexion instability of the knee after TKA. Human knee joint capsule tissues were collected at the time of primary or revision TKAs and analyzed by RT-qPCR and RNA-seq, revealing novel patterns of differential gene expression between the two groups. Interestingly, genes related to collagen production and extracellular matrix (ECM) degradation were higher in samples from patients with flexion instability. Partitioned clustering analyses further emphasized differential gene expression patterns between sample types that may help guide clinical interpretations of this complication. Future efforts to disentangle the effects of physical and biological (e.g., transcriptomic modifications) risk factors will aid in further characterizing and avoiding flexion instability after TKA.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1089-8646
Volume :
110
Issue :
5
Database :
MEDLINE
Journal :
Genomics
Publication Type :
Academic Journal
Accession number :
29174847
Full Text :
https://doi.org/10.1016/j.ygeno.2017.11.001