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Pro-inflammatory Analysis of Macrophages in Contact with Titanium Particles and Porphyromonas gingivalis.
- Source :
-
Brazilian dental journal [Braz Dent J] 2017 Jul-Aug; Vol. 28 (4), pp. 428-434. - Publication Year :
- 2017
-
Abstract
- During insertion of titanium dental implants, particles may shear from the implant to the periimplant region causing osteolysis, and their association with bacteria can exacerbate the inflammatory reaction. However, the association of a high invasive bacterium from the oral cavity, Porphyromonas gingivalis (Pg), and titanium particles remains unknown. This study evaluated pro-inflammatory reaction of human macrophages in contact with micro and nanoparticles of titanium associated with Porphyromonas gingivalis lipopolysaccharide (PgLPS). THP-1 cell were used and treated for 12, 24 and 48 h following 6 groups: Control(C), PgLPS (L); Microparticles (M); Nanoparticles (N); PgLPS and microparticles (LM); PgLPS and nanoparticles (LN). The following assays were carried out: i) cell viability using MTS, ii) cell morphology by SEM and iii) expression of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) by qRT-PCR and ELISA. For statistics two-way ANOVA followed by Tukey's test was used (p<0.05). After treatment, cells presented similar viability and morphology demonstrating that the treatments were not able to induce cell death. Gene expression was significantly higher for TNF-α and IL1-β after 12 h, and for IL-6 after 24 h in the N and LN groups. Cytokine production over time was an ascending curve for TNF-α with the peak at 48 h and IL1-β and IL-6 had a straight line among the time points, although cells from N group presented a significant production of IL-6 at 48 h. In conclusion, these results suggest that titanium nanoparticles stimulate stronger pro-inflammatory response in macrophages, independent of their association with LPS from P.gingivalis.
- Subjects :
- Cell Line
Cytokines genetics
Cytokines metabolism
Enzyme-Linked Immunosorbent Assay
Gene Expression
Humans
Inflammation Mediators metabolism
Macrophages metabolism
Microscopy, Electron, Scanning
O Antigens drug effects
Particle Size
Porphyromonas gingivalis immunology
Real-Time Polymerase Chain Reaction
Titanium chemistry
Dental Implants
Macrophages immunology
Porphyromonas gingivalis drug effects
Titanium pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1806-4760
- Volume :
- 28
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Brazilian dental journal
- Publication Type :
- Academic Journal
- Accession number :
- 29160393
- Full Text :
- https://doi.org/10.1590/0103-6440201701382