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Changes in gene expression in small bowel neuroendocrine tumors associated with progression to metastases.
- Source :
-
Surgery [Surgery] 2018 Jan; Vol. 163 (1), pp. 232-239. Date of Electronic Publication: 2017 Nov 16. - Publication Year :
- 2018
-
Abstract
- Background: Small bowel neuroendocrine tumors (SBNETs) present frequently with metastases, yet little is known about the molecular basis of this progression. This study sought to identify the serial differential expression of genes between normal small bowel, primary small bowel neuroendocrine tumors, and liver metastases.<br />Methods: RNA isolated from matched normal small bowel tissue, primary small bowel neuroendocrine tumors, and liver metastases in 12 patients was analyzed with whole transcriptome expression microarrays and RNA-Seq. Changes in gene expression between primary small bowel neuroendocrine tumors and normal small bowels, and liver metastases versus primary small bowel neuroendocrine tumors were calculated. Common genes that were differentially expressed serially (increasing or decreasing from normal small bowel to primary small bowel neuroendocrine tumors to liver metastases) were identified, and 10 were validated using qPCR.<br />Results: Use of 2 transcriptome platforms allowed for a robust discrimination of genes important in small bowel neuroendocrine tumors progression. Serial differential expression was validated in 7/10 genes, all of which had been described previously in abdominal cancers, and with several interacting with members of the AKT, MYC, or MAPK3 pathways. Liver metastases had consistent underexpression of PMP22, while high expression of SERPINA10 and SYT13 was characteristic of both pSBTs and liver metastases.<br />Conclusion: Identification of the serial differential expression of genes from normal tissues to primary tumors to metastases lends insight into important pathways for SBNETs progression. Differential expression of various genes, including PMP22, SYT13 and SERPINA10, are associated with the progression of SBNETs and warrant further investigation.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Subjects :
- Gene Expression
Gene Expression Profiling
Humans
Intestinal Neoplasms pathology
Intestine, Small metabolism
Liver Neoplasms secondary
Myelin P2 Protein metabolism
Neoplasm Metastasis
Neuroendocrine Tumors secondary
Sequence Analysis, RNA
Serpins metabolism
Synaptotagmins metabolism
Intestinal Neoplasms metabolism
Liver Neoplasms metabolism
Neuroendocrine Tumors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1532-7361
- Volume :
- 163
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Surgery
- Publication Type :
- Academic Journal
- Accession number :
- 29154080
- Full Text :
- https://doi.org/10.1016/j.surg.2017.07.031