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Oxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 release.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2018 Jan 01; Vol. 495 (1), pp. 1356-1362. Date of Electronic Publication: 2017 Nov 14. - Publication Year :
- 2018
-
Abstract
- Patients with ankylosing spondylitis (AS) have an increased risk for cardiovascular mortality. The circulating ox-LDL/LDL ratio is associated with subclinical atherosclerosis in patients with systemic lupus erythematosus. In this study, we found that the ox-LDL/LDL ratio was increased in AS patients. The levels of serum RANKL and HMGB1 were also elevated in AS patients, and the number of CD68 <superscript>+</superscript> /RANK <superscript>+</superscript> cells was increased in peripheral blood from AS patients. 0.03% ox-LDL in LDL, similar to the ox-LDL/LDL ratio in peripheral blood from AS patients, promoted cytoplasmic translocation and release of HMGB1 as well as RANK expression. Further investigation evidenced that ox-LDL-induced EGR1 expression contributed to the cytoplasmic translocation of HMGB1 and CD68 assisted the secretion of HMGB1 from cytoplasm to extracellular matrix. Extracellular HMGB1 induced RANK expression in CD68 <superscript>+</superscript> mononuclear cells, forming osteoclast precursors that were differentiated to osteoclasts in response to RANKL. Taken together, these results suggested that the changes, including ox-LDL/LDL ratio, CD68 <superscript>+</superscript> /RANK <superscript>+</superscript> cells number, and the levels of RANKL and HMGB1 in AS patients, favored osteoclastogenesis.<br /> (Copyright © 2017. Published by Elsevier Inc.)
- Subjects :
- Cell Differentiation immunology
Gene Expression Regulation immunology
Humans
Leukocytes, Mononuclear pathology
Spondylitis, Ankylosing pathology
Antigens, CD immunology
Antigens, Differentiation, Myelomonocytic immunology
HMGB1 Protein immunology
Leukocytes, Mononuclear immunology
Lipoproteins, LDL immunology
Osteogenesis immunology
RANK Ligand immunology
Spondylitis, Ankylosing immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 495
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 29146189
- Full Text :
- https://doi.org/10.1016/j.bbrc.2017.11.083