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Specific Hypersensitive Response-Associated Recognition of New Apoplastic Effectors from Cladosporium fulvum in Wild Tomato.
- Source :
-
Molecular plant-microbe interactions : MPMI [Mol Plant Microbe Interact] 2018 Jan; Vol. 31 (1), pp. 145-162. Date of Electronic Publication: 2017 Nov 16. - Publication Year :
- 2018
-
Abstract
- Tomato leaf mold disease is caused by the biotrophic fungus Cladosporium fulvum. During infection, C. fulvum produces extracellular small secreted protein (SSP) effectors that function to promote colonization of the leaf apoplast. Resistance to the disease is governed by Cf immune receptor genes that encode receptor-like proteins (RLPs). These RLPs recognize specific SSP effectors to initiate a hypersensitive response (HR) that renders the pathogen avirulent. C. fulvum strains capable of overcoming one or more of all cloned Cf genes have now emerged. To combat these strains, new Cf genes are required. An effectoromics approach was employed to identify wild tomato accessions carrying new Cf genes. Proteomics and transcriptome sequencing were first used to identify 70 apoplastic in planta-induced C. fulvum SSPs. Based on sequence homology, 61 of these SSPs were novel or lacked known functional domains. Seven, however, had predicted structural homology to antimicrobial proteins, suggesting a possible role in mediating antagonistic microbe-microbe interactions in planta. Wild tomato accessions were then screened for HR-associated recognition of 41 SSPs, using the Potato virus X-based transient expression system. Nine SSPs were recognized by one or more accessions, suggesting that these plants carry new Cf genes available for incorporation into cultivated tomato.
- Subjects :
- Alleles
Amino Acid Sequence
Cladosporium chemistry
Cladosporium genetics
Fungal Proteins genetics
Gene Expression Regulation, Fungal
Genes, Fungal
Proteomics
Repetitive Sequences, Nucleic Acid genetics
Sequence Analysis, RNA
Transcriptome genetics
Cladosporium metabolism
Fungal Proteins metabolism
Solanum lycopersicum immunology
Solanum lycopersicum microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0894-0282
- Volume :
- 31
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular plant-microbe interactions : MPMI
- Publication Type :
- Academic Journal
- Accession number :
- 29144204
- Full Text :
- https://doi.org/10.1094/MPMI-05-17-0114-FI