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Investigation of alimentary canal ultrastructure following knockdown of the Dicer-2 gene in planthoppers reveals the potential pathogenicity of southern rice black streaked dwarf virus to its insect vector.
- Source :
-
Virus research [Virus Res] 2018 Jan 15; Vol. 244, pp. 117-127. Date of Electronic Publication: 2017 Nov 12. - Publication Year :
- 2018
-
Abstract
- An increasing number of studies are suggesting that plant viruses, including southern rice black-streaked dwarf virus (SRBSDV), can adversely affect biological characteristics of insect vectors by unknown mechanisms. To study the adverse effect of SRBSDV at cellular level on the insect vector, we promoted viral infection by the disruption of the small interfering RNA (siRNA) pathway. The transmission electron microscopy was utilized to describe the ultrastructural changes that occurred in insects when the core component of the siRNA pathway, Dicer-2, was knocked down. The increasing accumulation of SRBSDV in virus-infected vector, the white-backed planthoppers, caused severe cytopathology in the alimentary canal. Similar cytopathology changes in the midgut ultrastructure were characterized in the virus-infected incompetent vector, the small brown planthopper. These results not only add support to the existing evidence suggesting that the siRNA pathway has an antiviral effect, but also reveal the universal and potential ability of SRBSDV to cause damage to the insect tissues of both the vector and non-vector.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Gastrointestinal Tract pathology
Gastrointestinal Tract ultrastructure
Hemiptera ultrastructure
Insect Proteins genetics
Insect Proteins metabolism
Insect Vectors ultrastructure
Microscopy, Electron, Transmission
Microvilli pathology
Microvilli ultrastructure
Microvilli virology
Oryza virology
Plant Diseases virology
Plant Viruses growth & development
RNA, Small Interfering genetics
RNA, Small Interfering metabolism
Ribonuclease III genetics
Ribonuclease III metabolism
Virulence
Gastrointestinal Tract virology
Hemiptera virology
Insect Proteins antagonists & inhibitors
Insect Vectors virology
Plant Viruses pathogenicity
Ribonuclease III antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7492
- Volume :
- 244
- Database :
- MEDLINE
- Journal :
- Virus research
- Publication Type :
- Academic Journal
- Accession number :
- 29141205
- Full Text :
- https://doi.org/10.1016/j.virusres.2017.11.012