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Direct reprogramming of mouse fibroblasts into neural cells via Porphyra yezoensis polysaccharide based high efficient gene co-delivery.
- Source :
-
Journal of nanobiotechnology [J Nanobiotechnology] 2017 Nov 14; Vol. 15 (1), pp. 82. Date of Electronic Publication: 2017 Nov 14. - Publication Year :
- 2017
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Abstract
- Background: The cell source for transplantation therapy is always a prerequisite question to be solved in clinical applications. Neural cells are considered non-regenerable, which highly restrict their application in the treatment for nerve injury. Therefore, neural trans-differentiation based on gene transfection provides a new solution to this issue. Compared to viral strategy, non-viral gene delivery systems are considered as a more promising way to achieve this aim. This study centers on a novel application of Porphyra yezoensis polysaccharide as a non-viral gene carrier for the neural trans-differentiation of mouse fibroblasts.<br />Results: Ethanediamine modified P. yezoensis polysaccharide (Ed-PYP) served as a gene carrier and a group of plasmids that encode Ascl1, Brn4, and Tcf3 (pABT) self-assembled into nanoparticles. Results demonstrated that Ed-PYP-pABT nanoparticles at Ed-PYP: pABT weight ratio of 40:1 was the optimal candidate for gene delivery. ELISA assay revealed the highest expression levels of NGF, BDNF and SHH at 14 days after last transfection. Immunofluorescence and western blot assays also showed robust expression of neural markers including Nestin, GFAP, β-3tubulin, NF200, GAP43 and MAP2, in induced 3T6 cells at this time point.<br />Conclusion: Overall, these findings indicated that the P. yezoensis polysaccharide-based non-viral gene co-delivery system is a promising strategy for the generation of neural cells, which might facilitate the developments in the recovery of neural injuries.
- Subjects :
- Animals
Basic Helix-Loop-Helix Transcription Factors genetics
Basic Helix-Loop-Helix Transcription Factors metabolism
Biomarkers metabolism
Brain-Derived Neurotrophic Factor genetics
Brain-Derived Neurotrophic Factor metabolism
Cell Transdifferentiation drug effects
Cellular Reprogramming drug effects
Ethylenediamines chemistry
Fibroblasts cytology
Fibroblasts metabolism
Gene Expression
Hedgehog Proteins genetics
Hedgehog Proteins metabolism
Mice
Nerve Growth Factor genetics
Nerve Growth Factor metabolism
Nerve Tissue Proteins genetics
Nerve Tissue Proteins metabolism
Neurons cytology
Neurons metabolism
POU Domain Factors genetics
POU Domain Factors metabolism
Plasmids chemistry
Plasmids metabolism
Polysaccharides chemistry
Polysaccharides isolation & purification
Fibroblasts drug effects
Gene Transfer Techniques
Nanoparticles
Neurons drug effects
Polysaccharides pharmacology
Porphyra chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1477-3155
- Volume :
- 15
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of nanobiotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 29137640
- Full Text :
- https://doi.org/10.1186/s12951-017-0317-y