Back to Search
Start Over
Vaccaria hypaphorine impairs RANKL-induced osteoclastogenesis by inhibition of ERK, p38, JNK and NF-κB pathway and prevents inflammatory bone loss in mice.
- Source :
-
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2018 Jan; Vol. 97, pp. 1155-1163. Date of Electronic Publication: 2017 Nov 11. - Publication Year :
- 2018
-
Abstract
- Osteoclasts are sole bone-resorbing cells which exert a profound effect on skeletal metabolism. The search for medicines that affect the differentiation and function of osteoclasts is crucial in developing therapies for osteoclast-based diseases. Vaccaria hypaphorine, the main active compound of the traditionally used Chinese herb Vaccaria segetalis, has anti-inflammatory activity. The present study demonstrated for the first time that vaccaria hypaphorine could significantly inhibit the receptor activator of nuclear factor kappa B ligand (RANKL)-induced osteoclastic differentiation in vitro and alleviate lipopolysaccharide (LPS)-induced bone loss in vivo. Further study showed that vaccaria hypaphorine decreased osteoclastogenesis in a dose-dependent manner. Furthermore, vaccaria hypaphorine was confirmed to inhibit osteoclasts differentiation at early stage but not at later stage. Pit formation assay and F-actin ring staining showed that vaccaria hypaphorine inhibited the bone-resorbing activity of osteoclasts. Mechanistically, vaccaria hypaphorine impaired RANKL-induced osteoclastogenesis through reduction of extracellular signal-regulated kinases (ERK), p38, c-Jun N-terminal kinase (JNK) and NF-κB p65 phosphorylation. Taken together, our results provided evidences that vaccaria hypaphorine might be considered as potential therapeutic agent for treating osteoclast-based bone loss.<br /> (Copyright © 2017 Elsevier Masson SAS. All rights reserved.)
- Subjects :
- Animals
Anti-Inflammatory Agents administration & dosage
Anti-Inflammatory Agents isolation & purification
Anti-Inflammatory Agents pharmacology
Bone Resorption drug therapy
Cell Differentiation drug effects
Dose-Response Relationship, Drug
Lipopolysaccharides
Male
Mice
Mice, Inbred C57BL
Plant Extracts administration & dosage
RANK Ligand administration & dosage
RANK Ligand metabolism
RAW 264.7 Cells
Osteoclasts drug effects
Osteogenesis drug effects
Plant Extracts pharmacology
Vaccaria chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1950-6007
- Volume :
- 97
- Database :
- MEDLINE
- Journal :
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
- Publication Type :
- Academic Journal
- Accession number :
- 29136954
- Full Text :
- https://doi.org/10.1016/j.biopha.2017.11.044