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Profiling the transcriptome with RNA SPOTs.

Authors :
Eng CL
Shah S
Thomassie J
Cai L
Source :
Nature methods [Nat Methods] 2017 Dec; Vol. 14 (12), pp. 1153-1155. Date of Electronic Publication: 2017 Nov 13.
Publication Year :
2017

Abstract

Single-molecule FISH (smFISH) has been the gold standard for quantifying individual transcript abundances. Here, we scale up multiplexed smFISH to the transcriptome level and profile 10,212 different mRNAs from mouse fibroblast and embryonic stem cells. This method, called RNA sequential probing of targets (SPOTs), provides an accurate, flexible, and low-cost alternative to sequencing for profiling transcriptomes.

Subjects

Subjects :
Animals
Embryonic Stem Cells
Fibroblasts
High-Throughput Nucleotide Sequencing
Mice
NIH 3T3 Cells
Reproducibility of Results
Sensitivity and Specificity
Sequence Analysis, RNA
Gene Expression Profiling methods
In Situ Hybridization, Fluorescence methods
RNA Probes
RNA, Messenger genetics

Details

Language :
English
ISSN :
1548-7105
Volume :
14
Issue :
12
Database :
MEDLINE
Journal :
Nature methods
Publication Type :
Academic Journal
Accession number :
29131163
Full Text :
https://doi.org/10.1038/nmeth.4500
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