Back to Search
Start Over
Visualization of lipid directed dynamics of perilipin 1 in human primary adipocytes.
- Source :
-
Scientific reports [Sci Rep] 2017 Nov 08; Vol. 7 (1), pp. 15011. Date of Electronic Publication: 2017 Nov 08. - Publication Year :
- 2017
-
Abstract
- Perilipin 1 is a lipid droplet coating protein known to regulate lipid metabolism in adipocytes by serving as a physical barrier as well as a recruitment site for lipases to the lipid droplet. Phosphorylation of perilipin 1 by protein kinase A rapidly initiates lipolysis, but the detailed mechanism on how perilipin 1 controls lipolysis is unknown. Here, we identify specific lipid binding properties of perilipin 1 that regulate the dynamics of lipolysis in human primary adipocytes. Cellular imaging combined with biochemical and biophysical analyses demonstrate that perilipin 1 specifically binds to cholesteryl esters, and that their dynamic properties direct segregation of perilipin 1 into topologically distinct micro domains on the lipid droplet. Together, our data points to a simple unifying mechanism that lipid assembly and segregation control lipolysis in human primary adipocytes.
- Subjects :
- Adipocytes cytology
Cells, Cultured
Cyclic AMP-Dependent Protein Kinases metabolism
Humans
Lipase metabolism
Lipolysis
Membrane Microdomains metabolism
Microscopy, Confocal
Microscopy, Fluorescence
Phosphorylation
Protein Binding
Sterol Esterase metabolism
Adipocytes metabolism
Lipid Droplets metabolism
Lipid Metabolism
Perilipin-1 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 29118433
- Full Text :
- https://doi.org/10.1038/s41598-017-15059-4