Back to Search
Start Over
Cholesterol-α-glucosyltransferase gene is present in most Helicobacter species including gastric non-Helicobacter pylori helicobacters obtained from Japanese patients.
- Source :
-
Helicobacter [Helicobacter] 2018 Feb; Vol. 23 (1). Date of Electronic Publication: 2017 Nov 07. - Publication Year :
- 2018
-
Abstract
- Background: Non-Helicobacter pylori helicobacters (NHPHs) besides H. pylori infect human stomachs and cause chronic gastritis and mucosa-associated lymphoid tissue lymphoma. Cholesteryl-α-glucosides have been identified as unique glycolipids present in H. pylori and some Helicobacter species. Cholesterol-α-glucosyltransferase (αCgT), a key enzyme for the biosynthesis of cholesteryl-α-glucosides, plays crucial roles in the pathogenicity of H. pylori. Therefore, it is important to examine αCgTs of NHPHs.<br />Materials and Methods: Six gastric NHPHs were isolated from Japanese patients and maintained in mouse stomachs. The αCgT genes were amplified by PCR and inverse PCR. We retrieved the αCgT genes of other Helicobacter species by BLAST searches in GenBank.<br />Results: αCgT genes were present in most Helicobacter species and in all Japanese isolates examined. However, we could find no candidate gene for αCgT in the whole genome of Helicobacter cinaedi and several enterohepatic species. Phylogenic analysis demonstrated that the αCgT genes of all Japanese isolates show high similarities to that of a zoonotic group of gastric NHPHs including Helicobacter suis, Helicobacter heilmannii, and Helicobacter ailurogastricus. Of 6 Japanese isolates, the αCgT genes of 4 isolates were identical to that of H. suis, and that of another 2 isolates were similar to that of H. heilmannii and H. ailurogastricus.<br />Conclusions: All gastric NHPHs examined showed presence of αCgT genes, indicating that αCgT may be beneficial for these helicobacters to infect human and possibly animal stomachs. Our study indicated that NHPHs could be classified into 2 groups, NHPHs with αCgT genes and NHPHs without αCgT genes.<br /> (© 2017 John Wiley & Sons Ltd.)
- Subjects :
- Animals
Female
Gastritis microbiology
Gastritis pathology
Genome, Bacterial genetics
Helicobacter classification
Helicobacter Infections pathology
Humans
Japan
Lymphoma, B-Cell, Marginal Zone pathology
Mice
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Sequence Alignment
Sequence Homology, Amino Acid
Virulence genetics
Glucosyltransferases genetics
Helicobacter enzymology
Helicobacter genetics
Helicobacter Infections microbiology
Lymphoma, B-Cell, Marginal Zone microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1523-5378
- Volume :
- 23
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Helicobacter
- Publication Type :
- Academic Journal
- Accession number :
- 29110387
- Full Text :
- https://doi.org/10.1111/hel.12449