Back to Search Start Over

Ammoides pusilla (Apiaceae) essential oil: Activity against Acanthamoeba castellanii Neff.

Authors :
Souhaiel N
Sifaoui I
Ben Hassine D
Bleton J
Bonose M
Moussa F
Piñero JE
Lorenzo-Morales J
Abderrabba M
Source :
Experimental parasitology [Exp Parasitol] 2017 Dec; Vol. 183, pp. 99-103. Date of Electronic Publication: 2017 Nov 02.
Publication Year :
2017

Abstract

Acanthamoeba is a free-living amoeba genus that causes several diseases namely, amoebic keratitis which is a painful sight threatening eyes disease. Its treatment is difficult and the exploration for new drugs is very important. The main objective of the present study was to evaluate the chemical composition of the Essential Oils (EO) obtained from leaves and flowers and aerial parts of Ammoides pusilla by an alternative method "Hydrodistillation''. Identification and quantification were realized by Gas Chromatography-Mass Spectrometry (GC-MS) and Gas Chromatography with Flame Ionization Detection (GC-FID). The main components of leaves and flowers and aerials parts were thymol (39.6% and 33.05%), γ-terpinene (28.97% and 28.19%), p-cymene (13.69% and 15.31%) and thymol methyl ether (7.33% and 8.91%), respectively. The antiparasitic activity of the EO was evaluated against Acanthamoeba castellanii Neff by the Alamar Blue <superscript>®</superscript> assay. Results showed that Ammoides pusilla amoebicidal activity from leaves and flowers essential oil (IC <subscript>50</subscript>  = 65.32 ± 5.43 μg/mL) was more important than those of aerial parts EO (IC <subscript>50</subscript>  = 97.18 ± 1.43 μg/ml).<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1090-2449
Volume :
183
Database :
MEDLINE
Journal :
Experimental parasitology
Publication Type :
Academic Journal
Accession number :
29102680
Full Text :
https://doi.org/10.1016/j.exppara.2017.10.011