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Localization of neuroglobin in the brain of R6/2 mouse model of Huntington's disease.
- Source :
-
Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology [Neurol Sci] 2018 Feb; Vol. 39 (2), pp. 275-285. Date of Electronic Publication: 2017 Nov 03. - Publication Year :
- 2018
-
Abstract
- Neuroglobin (Ngb) is expressed in the central and peripheral nervous system, cerebrospinal fluid, retina, and endocrine tissues where it is involved in binding O <subscript>2</subscript> and other gasotransmitters. Several studies have highlighted its endogenous neuroprotective function. Huntington's disease (HD), a dominant hereditary disease, is characterized by the gradual loss of neurons in discrete areas of the central nervous system. We analyzed the expression of Ngb in the brain tissue of a mouse model of HD, in order to define the role of Ngb with respect to individual cell type vulnerability in HD and to gender and age of mice. Our results showed different expressions of Ngb among neurons of a specific region and between different brain regions. We evidenced a decreased intensity of Ngb at 13 weeks of age, compared to 7 weeks of age. The double immunofluorescence and fluorescence resonance energy transfer (FRET) experiments showed that the co-localization between Ngb and huntingtin at the subcellular level was not close enough to account for a direct interaction. We also observed a different expression of Ngb in the striatum, depending on the sex and age of animals. These findings provide the first experimental evidence for an adaptive response of Ngb in HD, suggesting that Ngb may exert neuroprotective effects in HD beyond its role in reducing sensitivity to oxidative stress.
- Subjects :
- ADP-Ribosylation Factors
Animals
Bacterial Toxins
Cell Line, Tumor
Cholinesterases metabolism
Corpus Striatum pathology
Disease Models, Animal
Female
Fluorescence Resonance Energy Transfer
Huntingtin Protein genetics
Huntington Disease genetics
Male
Mice
Mice, Transgenic
Mutation genetics
Neuroglobin
Neurons metabolism
Parvalbumins metabolism
Sex Factors
Time Factors
Corpus Striatum metabolism
Gene Expression Regulation genetics
Globins metabolism
Huntington Disease pathology
Nerve Tissue Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1590-3478
- Volume :
- 39
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology
- Publication Type :
- Academic Journal
- Accession number :
- 29101592
- Full Text :
- https://doi.org/10.1007/s10072-017-3168-2