Back to Search
Start Over
Serological tests for gambiense human African trypanosomiasis detect antibodies in cattle.
- Source :
-
Parasites & vectors [Parasit Vectors] 2017 Nov 03; Vol. 10 (1), pp. 546. Date of Electronic Publication: 2017 Nov 03. - Publication Year :
- 2017
-
Abstract
- Background: Serological tests for gambiense human African trypanosomiasis (gHAT) detect antibodies to antigens on the cell surface of bloodstream trypanosomes. As trypanosomes that cause animal African trypanosomiasis (AAT) also express related antigens, we have evaluated two rapid diagnostic tests (RDTs) on cattle in trypanosomiasis endemic and non-endemic regions, to determine whether gHAT serological tests could also be used to screen for AAT.<br />Methods: Two RDTs, 1G RDT, made with native antigens, and p2G RDT, made with recombinant antigens, were tested on 121 cattle in a trypanosomiasis-free region, and on 312 cattle from a rhodesiense HAT and AAT endemic region. A subset of samples from the endemic region were also tested with two immune trypanolysis (TL) tests. The sensitivity of the tests was estimated by evaluating the result of the RDT on samples that were positive by both microscopy and internal transcribed spacer (ITS) PCR, whilst specificity was the result of the RDT on samples that were negative by ITS PCR and microscopy, and others from the non-endemic region.<br />Results: The specificity of the p2G RDT on cattle from the non-endemic region was 97.5% (95% CI: 93.0-99.2%), compared to only 57.9% (95% CI: 48.9-66.3%) for 1G RDT. The specificities of 1G RDT, p2G RDT and TL on endemic control cattle were 14.6% (95% CI: 9.7-21.5%), 22.6% (95% CI: 16.4-30.3%) and 68.3% (95% CI: 59.6-75.9%), respectively. The sensitivities of the tests on trypanosome positive samples were 85.1% (95% CI: 79.1-89.7%), 89.1% (95% CI: 83.7-93.0%) and 59.3% (95% CI: 51.8-66.4%), respectively. Among the same samples, 51.7% were positive by both TL and the 1G RDT.<br />Conclusions: These serological tests detect cross-reacting antibodies in cattle. The p2G RDT based on recombinant antigens had a high specificity in a non-endemic region, while the 1G RDT had a lower specificity, suggesting cross-reactivity with other pathogens.
- Subjects :
- Animals
Antigens, Protozoan immunology
Cattle
Cattle Diseases epidemiology
Cattle Diseases immunology
Cross Reactions
Humans
Polymerase Chain Reaction
Sensitivity and Specificity
Trypanosomiasis, African diagnosis
Trypanosomiasis, African epidemiology
Trypanosomiasis, African immunology
Antibodies, Protozoan blood
Cattle Diseases diagnosis
Serologic Tests
Trypanosoma brucei gambiense immunology
Trypanosomiasis, African veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1756-3305
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Parasites & vectors
- Publication Type :
- Academic Journal
- Accession number :
- 29100526
- Full Text :
- https://doi.org/10.1186/s13071-017-2487-8