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A disposable microfluidic device with a reusable magnetophoretic functional substrate for isolation of circulating tumor cells.
- Source :
-
Lab on a chip [Lab Chip] 2017 Nov 21; Vol. 17 (23), pp. 4113-4123. - Publication Year :
- 2017
-
Abstract
- We describe an assembly-disposable microfluidic device based on a silicone-coated release polymer thin film. It consists of a disposable polymeric superstrate and a reusable functional substrate and they are assembled simply using vacuum pressure. The disposable polymeric superstrate is manufactured by bonding a silicone-coated release polymer thin film and a microstructured polydimethylsiloxane (PDMS) replica, containing only a simple structured microchannel. The reusable functional substrate generates an intricate energy field that can penetrate the micrometer-thick polymer film into the microchannel and control microfluids. This is the first report to introduce a silicone-coated release polyethylene terephthalate (PET) thin film as a bonding layer on a microstructured PDMS replica. The bonding strength was ∼600 kPa, which is the strongest among bonding methods of PDMS and PET polymer. Additionally, accelerated tests for bond stability and leakage demonstrated that the silicone-coated release PET film can form a very robust bond with PDMS. To demonstrate the usefulness of the proposed assembly-disposable microfluidic device, a lateral magnetophoretic microseparator was developed in an assembly-disposable microfluidic device format and was evaluated for isolating circulating tumor cells (CTCs) from patients with breast cancer.
Details
- Language :
- English
- ISSN :
- 1473-0189
- Volume :
- 17
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- Lab on a chip
- Publication Type :
- Academic Journal
- Accession number :
- 29094741
- Full Text :
- https://doi.org/10.1039/c7lc00925a