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Erythropoietin reduces hippocampus injury in neonatal rats with hypoxic ischemic brain damage via targeting matrix metalloprotein-2.
- Source :
-
European review for medical and pharmacological sciences [Eur Rev Med Pharmacol Sci] 2017 Oct; Vol. 21 (19), pp. 4327-4333. - Publication Year :
- 2017
-
Abstract
- Objective: Erythropoietin (EPO), as a type of the tissue-protective cytokines, is a 30.4 kDa hematopoietic glycoprotein. The purpose of this study was to explore the neuroprotective effects of EPO on the neonatal hypoxic-ischemic-induced hippocampus injury and the MMP-2 expression.<br />Materials and Methods: Neonatal Sprague-Dawley (SD) rats were randomly divided into an untreated group (control) and two hypoxia-ischemia (HI) groups treated with saline control or EPO. Hippocampi were harvested at various times after return to normoxia (6 h, 24 h, 3 days and 7 days post-HI) for analyses of infarct areas and expression using histology, Western blot and reverse transcriptase-polymerase chain reaction (RT-PCR).<br />Results: EPO injections reduced the infarction and loss of brain tissue. HI group exhibited an enhanced MMP-2 positive staining compared to controls at 24 h, 3 and 7 days post-HI by immunohistochemistry. These results were confirmed by Western blot analysis of MMP-2 expression at 7 days post-HI. Levels of MMP-2 mRNA in the injured hippocampi increased significantly at 24 h and 7 days post-HI. In particular, the EPO treatment further significantly enhanced this increase.<br />Conclusions: EPO protected hypoxic-ischemic-induced neonatal brain damage by up-regulating the MMP-2 expression. Hence, systemic EPO may have potential utility for the treatment of HI injury in human newborns.
- Subjects :
- Animals
Animals, Newborn
Cerebral Infarction pathology
Cerebral Infarction prevention & control
Male
Matrix Metalloproteinase 2 biosynthesis
Rats
Rats, Sprague-Dawley
Up-Regulation drug effects
Erythropoietin therapeutic use
Hippocampus pathology
Hypoxia-Ischemia, Brain drug therapy
Hypoxia-Ischemia, Brain pathology
Matrix Metalloproteinase 2 drug effects
Neuroprotective Agents therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 2284-0729
- Volume :
- 21
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- European review for medical and pharmacological sciences
- Publication Type :
- Academic Journal
- Accession number :
- 29077163