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Reg Gene Expression in Periosteum after Fracture and Its In Vitro Induction Triggered by IL-6.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2017 Oct 27; Vol. 18 (11). Date of Electronic Publication: 2017 Oct 27. - Publication Year :
- 2017
-
Abstract
- The periosteum is a thin membrane that surrounds the outer surface of bones and participates in fracture healing. However, the molecular signals that trigger/initiate the periosteal reaction are not well established. We fractured the rat femoral bone at the diaphysis and fixed it with an intramedullary inserted wire, and the expression of regenerating gene ( Reg ) I , which encodes a tissue regeneration/growth factor, was analyzed. Neither bone/marrow nor muscle showed Reg I gene expression before or after the fracture. By contrast, the periosteum showed an elevated expression after the fracture, thereby confirming the localization of Reg I expression exclusively in the periosteum around the fractured areas. Expression of the Reg family increased after the fracture, followed by a decrease to basal levels by six weeks, when the fracture had almost healed. In vitro cultures of periosteal cells showed no Reg I expression, but the addition of IL-6 significantly induced Reg I gene expression. The addition of IL-6 also increased the cell number and reduced pro-apoptotic gene expression of Bim . The increased cell proliferation and reduction in Bim gene expression were abolished by transfection with Reg I siRNA, indicating that these IL-6-dependent effects require the Reg I gene expression. These results indicate the involvement of the IL-6/Reg pathway in the osteogenic response of the periosteum, which leads to fracture repair.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Animals
Apoptosis genetics
Bcl-2-Like Protein 11 genetics
Bcl-2-Like Protein 11 metabolism
Cells, Cultured
Disease Models, Animal
Fractures, Bone pathology
Gene Expression Profiling
Immunohistochemistry
Lithostathine metabolism
Male
Mesenchymal Stem Cells metabolism
Periosteum cytology
Periosteum pathology
RNA, Messenger genetics
RNA, Messenger metabolism
Rats
Fracture Healing
Fractures, Bone genetics
Fractures, Bone metabolism
Gene Expression Regulation
Interleukin-6 metabolism
Lithostathine genetics
Periosteum metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 18
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 29077068
- Full Text :
- https://doi.org/10.3390/ijms18112257