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Antifungal activity and patterns of N-acetyl-chitooligosaccharide degradation via chitinase produced from Serratia marcescens PRNK-1.
- Source :
-
Microbial pathogenesis [Microb Pathog] 2017 Dec; Vol. 113, pp. 218-224. Date of Electronic Publication: 2017 Oct 23. - Publication Year :
- 2017
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Abstract
- Serratia marcescens PRNK-1, which has strong chitinolytic activity, was isolated from cockroaches (Periplaneta americana L.). The chitinase from S. marcescens PRNK-1 was characterized after incubation in a 0.5% colloidal chitin medium at 30 °C for 3 days. The molecular weights of three bands after staining for chitinase activity were approximately 34, 41, and 48 kDa on an SDS-PAGE gel. S. marcescens PRNK-1 strain strongly inhibited hyphal growth of Rhizoctonia solani and Fusarium oxysporum. Thin-layer chromatography (TLC) and high performance liquid chromatograph (HPLC) analyses were conducted to investigate the degradation patterns of N-acetyl-chitooligosaccharides by PRNK-1 chitinase. The N-acetyl-chitooligosaccharides: N-acetyl-chitin dimer (GlcNAc) <subscript>2</subscript> , N-acetyl-chitin trimer (GlcNAc) <subscript>3</subscript> , and N-acetyl-chitin tetramer (GlcNAc) <subscript>4</subscript> were degraded to (GlcNAc) <subscript>1-3</subscript> on a TLC plate. In an additional experiment, (GlcNAc) <subscript>6</subscript> was degraded to (GlcNAc) <subscript>1-4</subscript> on a TLC plate. The optimal temperature for chitinase activity of the PRNK-1 was 50 °C, producing 32.8 units/mL. As seen via TLC, the highest degradation of (GlcNAc) <subscript>4</subscript> by PRNK-1 chitinase occurred with 50 °C incubation. The optimal pH for chitinase activity of PRNK-1 was pH 5.5, producing 24.6 units/mL. As seen via TLC, the highest degradation of (GlcNAc) <subscript>4</subscript> by PRNK-1 chitinase occurred at pH 5.0-6.0. These results indicate that chitinase produced from S. marcescens PRNK-1 strain showed strong antifungal activity and potential of production of N-acetyl-chitooligosaccharides.<br /> (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Chitin chemistry
Chitin metabolism
Chitinases chemistry
Chitinases isolation & purification
Chitosan
Cockroaches microbiology
Enzyme Assays
Enzyme Stability
Fusarium drug effects
Hydrogen-Ion Concentration
Hyphae drug effects
Hyphae growth & development
Methyltransferases
Molecular Weight
Oligosaccharides
Phylogeny
Rhizoctonia drug effects
Serratia marcescens classification
Serratia marcescens genetics
Serratia marcescens isolation & purification
Temperature
Antifungal Agents pharmacology
Chitin analogs & derivatives
Chitinases metabolism
Chitinases pharmacology
Serratia marcescens enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-1208
- Volume :
- 113
- Database :
- MEDLINE
- Journal :
- Microbial pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 29074434
- Full Text :
- https://doi.org/10.1016/j.micpath.2017.10.039