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The downregulation of miR-3173 in B-cell acute lymphoblastic leukaemia promotes cell invasion via PTK2.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2017 Dec 16; Vol. 494 (3-4), pp. 569-574. Date of Electronic Publication: 2017 Oct 21. - Publication Year :
- 2017
-
Abstract
- MicroRNAs are important regulators of the pathogenesis of B-cell acute lymphoblastic leukaemia (B-ALL). In this study, we identified miR-3173 and its predicted target gene PTK2 were correspondingly differentially expressed in B-ALL patients. In B-ALL cell lines, CCK-8 proliferation assay revealed that miR-3173 could inhibit the cell proliferation. Moreover, transwell assay revealed that miR-3173 could also inhibit cell migration and invasion in B-ALL cell lines. Luciferase assays revealed that miR-3173 directly bound to the 3'untranslated region of PTK2, and western blotting showed that miR-3173 suppressed the expression of PTK2 at the protein level. Generally, this study indicates that miR-3173 negatively regulates PTK2 and inhibits proliferation and invasion of B-ALL cell lines. Thus, miR-3173 may represent a potential therapeutic molecule for B-ALL intervention.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Subjects :
- Adolescent
Adult
Cell Movement
Child
Child, Preschool
Down-Regulation
Female
Humans
Infant
Male
Neoplasm Invasiveness
Tumor Cells, Cultured
Young Adult
Focal Adhesion Kinase 1 metabolism
MicroRNAs metabolism
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma metabolism
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 494
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 29066351
- Full Text :
- https://doi.org/10.1016/j.bbrc.2017.10.013